Carpenter M B, Huang Y, Pereira A B, Hersh L B
Department of Anatomy, F.E. Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
J Hirnforsch. 1990;31(5):585-99.
Immunocytochemical studies of the vestibular nuclei (VN) were done in the squirrel monkey and cat using polyclonal antisera. Brain stem sections were processed using the Avidin-Biotin peroxidase complex with diaminobenzidine as the chromagen. Choline acetyltransferase immunoreactivity (ChAT-IR) was most prevalent in the caudal medial (MVN), inferior (IVN) and peripheral superior (SVN) VN. Nearly all cells of groups x and z were ChAT-positive. None of the giant cells of the lateral vestibular nucleus (LVN) was ChAT-IR. Glutamate immunoreactivity (GLU-IR) was abundant in all VN and in cells of the vestibular ganglion (VG). Gamma-aminobutyric acid immunoreactivity (GABA-IR), was found in cells of rostral MVN, cell group y and in granules about giant cells in dorsal LVN. Substance P immunoreactive (SP-IR) was present in a small cells in MVN, IVN and the VG and in granules surrounding all large cells in LVN in both monkey and cat; SP-IR granules were most intense in ventral LVN in the monkey. Some cells in the dorsal parts of the fastigial nucleus (FN) were outlined by SP-IR granules in both species. Leucine-enkephalin immunoreactivity (ENK-IR) was identified only in granules surrounding cells of group x in the monkey. GLU was the only immunoreactive substance found in the giant cells of LVN. The disposition of ChAT-IR in the VN suggested participation in commissural systems, as well as projections to spinal cord and/or cerebellum. Small GABA-IR neurons in MVN probably represented both commissural and projection neurons; GABA-IR granules about cells in dorsal LVN and some cells in MVN and SVN appeared to represent Purkinje cell (PC) terminals. SP-IR granules surrounding cells in ventral LVN appeared to represent terminals of small SP-positive VG cells. The source of SP-IR granules around cells in dorsal LVN and some cells in FN and SVN remains unknown, but these fibers may originate from portions of the reticular formation known to contain large numbers of SP-positive neurons.
利用多克隆抗血清，对松鼠猴和猫的前庭核（VN）进行了免疫细胞化学研究。脑干切片采用抗生物素蛋白-生物素过氧化物酶复合物处理，以二氨基联苯胺作为显色剂。胆碱乙酰转移酶免疫反应性（ChAT-IR）在尾侧内侧（MVN）、下侧（IVN）和外周上侧（SVN）前庭核中最为普遍。几乎所有x组和z组的细胞都是ChAT阳性。外侧前庭核（LVN）的巨细胞均无ChAT-IR。谷氨酸免疫反应性（GLU-IR）在前庭核和前庭神经节（VG）的细胞中均很丰富。γ-氨基丁酸免疫反应性（GABA-IR）在前庭内侧核头侧、y细胞群以及背侧前庭核巨细胞周围的颗粒中被发现。P物质免疫反应性（SP-IR）存在于前庭内侧核、下侧前庭核和前庭神经节的小细胞中，以及猴和猫的外侧前庭核所有大细胞周围的颗粒中；猴的腹侧前庭核中SP-IR颗粒最为密集。在这两个物种中，顶核（FN）背侧部分的一些细胞被SP-IR颗粒勾勒出来。亮氨酸脑啡肽免疫反应性（ENK-IR）仅在猴的x组细胞周围的颗粒中被识别。谷氨酸是在外侧前庭核巨细胞中发现的唯一免疫反应性物质。前庭核中ChAT-IR的分布表明其参与连合系统，以及向脊髓和/或小脑的投射。前庭内侧核中的小GABA-IR神经元可能代表连合神经元和投射神经元；背侧前庭核细胞周围的GABA-IR颗粒以及前庭内侧核和上侧前庭核中的一些细胞似乎代表浦肯野细胞（PC）终末。腹侧前庭核细胞周围的SP-IR颗粒似乎代表小的SP阳性前庭神经节细胞的终末。背侧前庭核细胞以及顶核和上侧前庭核中一些细胞周围的SP-IR颗粒来源尚不清楚，但这些纤维可能起源于已知含有大量SP阳性神经元的网状结构部分。
