Tsukazaki K, Kobiki K, Hasegawa K, Sakayori M, Nozawa S
Department of Obstetrics and Gynecology, School of Medicine, Keio University.
Nihon Rinsho. 1992 Oct;50(10):2476-83.
In cytology, it is often difficult to make a definite diagnosis of endometrial cancer. In order to devise a new supplementary method, using flow cytometry we analyzed the reactivities of endometrial cells with two monoclonal antibodies (MSN-1 and MSN-3), both of which are strongly reactive to endometrial cancer cells. Employing either or both antibodies in flow cytometry, we investigated the usefulness of this analysis for distinguishing endometrial cancer cells from normal endometrial cells. Results revealed a low positive rate for normal endometrium: 9.2% with MSN-1 and 5.0% with MSN-3. In contrast, for endometrial cancer the positive rate was high: 81.2% with MSN-1 and 43.8% with MSN-3. Combined analysis with both antibodies increased the positive rate for endometrial cancer to 93.7%. These results suggest that analysis by flow cytometry employing MSN-1 and MSN-3 may have a diagnostic value for endometrial cancer. We are also investigating the usefulness of two-color analysis using these antibodies.
在细胞学中，对子宫内膜癌进行明确诊断往往很困难。为了设计一种新的辅助方法，我们使用流式细胞术分析了子宫内膜细胞与两种单克隆抗体（MSN-1和MSN-3）的反应性，这两种抗体均对子宫内膜癌细胞有强烈反应。在流式细胞术中使用其中一种或两种抗体，我们研究了这种分析方法在区分子宫内膜癌细胞与正常子宫内膜细胞方面的实用性。结果显示，正常子宫内膜的阳性率较低：MSN-1为9.2%，MSN-3为5.0%。相比之下，子宫内膜癌的阳性率较高：MSN-1为81.2%，MSN-3为43.8%。两种抗体联合分析使子宫内膜癌的阳性率提高到93.7%。这些结果表明，使用MSN-1和MSN-3的流式细胞术分析可能对子宫内膜癌具有诊断价值。我们也在研究使用这些抗体进行双色分析的实用性。
