使用两种单克隆抗体通过流式细胞术检测子宫内膜癌
Detection of endometrial cancer by flow cytometry using two monoclonal antibodies.
作者信息
Kobiki K, Tsukasaki K, Kubushiro K, Kobayashi Y, Hasegawa K, Fukuchi T, Banno K, Nozawa S
机构信息
Department of Obstetrics and Gynecology, Kawasaki Municipal Hospital, Kawasaki City, Japan.
出版信息
Cytometry. 1999 Jun 1;36(2):150-6. doi: 10.1002/(sici)1097-0320(19990601)36:2<150::aid-cyto9>3.0.co;2-j.
BACKGROUND
To develop a supplementary diagnostic method for endometrial cancer by measuring the reactivity of various endometrial lesions with two monoclonal antibodies.
METHODS
We investigated the reactivity of various endometrial lesions with two monoclonal antibodies (MSN-1 and MSN-3) by flow cytometry (one-color and two-color methods).
RESULTS
The two-color method appeared to be suitable for use in place of simultaneous performance of the one-color methods with MSN-1 and MSN-3. The positivity rate for normal endometrium was 16.0% with the two-color method, which was lower than the rate of 30.0% obtained with concomitant used of the one-color methods. The positivity rate for endometrial cancer was high, 84.0%, with the two-color method. The positivity rate was 85.7% for well-differentiated endometrial cancer, 71.4% for moderately differentiated cancer, and 100.0% for poorly differentiated cancer; thus, the rate was high irrespective of the cellular differentiation.
CONCLUSIONS
The two-color method is more useful than the one-color method as a supplementary diagnostic procedure for endometrial cancer.
背景
通过检测各种子宫内膜病变与两种单克隆抗体的反应性，开发一种子宫内膜癌的辅助诊断方法。
方法
我们采用流式细胞术（单色法和双色法）研究了各种子宫内膜病变与两种单克隆抗体（MSN-1和MSN-3）的反应性。
结果
双色法似乎适用于替代同时使用MSN-1和MSN-3的单色法。双色法检测正常子宫内膜的阳性率为16.0%，低于同时使用单色法时获得的30.0%的阳性率。双色法检测子宫内膜癌的阳性率较高，为84.0%。高分化子宫内膜癌的阳性率为85.7%，中分化癌为71.4%，低分化癌为100.0%；因此，无论细胞分化程度如何，阳性率都很高。
结论
作为子宫内膜癌的辅助诊断方法，双色法比单色法更有用。
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