Lee Hee-Kyung, Yeo Sang-Geon
College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Chinju 660-701, Republic of Korea.
Virus Genes. 2003;26(2):207-12. doi: 10.1023/a:1023447732567.
The nucleocapsid (N) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered diagnostic reagents. Also, sequences of the nucleotides and deduced amino acids of the Chinju99 N gene were analyzed by alignment with those of CV777 and Brl/87. The nucleotide sequence encoding the entire N gene open reading frame (ORF) of Chinju99 was 1326 bases long and encoded a protein of 441 amino acids with predicted M(r) of 49 kDa. It consisted of 405 adenine (30.5%), 293 cytosine (22.1%), 334 guanines (25.2%) and 294 thymines (22.2%) residues. The Chinju99 N ORF nucleotide sequence was 96.5% and 96.4% homologous with that of the CV777 and Brl/87, respectively. The Chinju99 N protein revealed 96.8% amino acid identity with that of Brl/87 and CV777, respectively. The amino acid sequence contained seven potential sites for threonine (T)- or serine (S)-linked phosphorylation by each protein kinase C and casein kinase II.
对先前在韩国晋州分离出的猪流行性腹泻病毒（PEDV）晋州99株的核衣壳（N）基因进行了克隆和测序，以建立用于开发基因工程诊断试剂的信息。此外，通过与CV777和Brl/87的核苷酸和推导氨基酸序列进行比对，对晋州99株N基因进行了分析。编码晋州99株完整N基因开放阅读框（ORF）的核苷酸序列长1326个碱基，编码一个441个氨基酸的蛋白质，预测分子量为49 kDa。它由405个腺嘌呤（30.5%）、293个胞嘧啶（22.1%）、334个鸟嘌呤（25.2%）和294个胸腺嘧啶（22.2%）残基组成。晋州99株N ORF核苷酸序列与CV777和Brl/87的核苷酸序列分别具有96.5%和96.4%的同源性。晋州99株N蛋白与Brl/87和CV777的氨基酸序列分别具有96.8%的同一性。该氨基酸序列包含七个潜在的苏氨酸（T）或丝氨酸（S）连接的磷酸化位点，分别由蛋白激酶C和酪蛋白激酶II催化。
