Yeo Sang-Geon, Hernandez Mercedes, Krell Peter J, Nagy Eva E
College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Chinju 660-701, Republic of Korea.
Virus Genes. 2003 May;26(3):239-46. doi: 10.1023/a:1024443112717.
The spike (S) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to aid in the development of genetically engineered vaccines and diagnostic reagents against PEDV. The nucleotide sequence encoding the entire S gene open reading frame (ORF) of Chinju99 was 4,152 bases long encoding 1,383 amino acids. It consisted of 1,001 adenine (24.1%), 849 cytosine (20.4%), 877 guanine (21.1%) and 1425 thymine (34.3%) residues. The Chinju99 S ORF nucleotide sequence was 94.5% homologous with that of the Br1/87 and CV777 strains, respectively. The Chinju99 S protein had 92.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained 27 potential sites for asparagine (N)-linked glycosylation and there was a stretch of highly hydrophobic residues at position 1,325-1,350.
之前在韩国晋州分离出的猪流行性腹泻病毒（PEDV）晋州99株的刺突（S）基因被克隆并测序，以助力开发针对PEDV的基因工程疫苗和诊断试剂。编码晋州99株整个S基因开放阅读框（ORF）的核苷酸序列长4152个碱基，编码1383个氨基酸。它由1001个腺嘌呤（24.1%）、849个胞嘧啶（20.4%）、877个鸟嘌呤（21.1%）和1425个胸腺嘧啶（34.3%）残基组成。晋州99株S ORF核苷酸序列与Br1/87株和CV777株的序列分别具有94.5%的同源性。晋州99株S蛋白与Br1/87株和CV777株的S蛋白分别具有92.8%的氨基酸同一性。氨基酸序列包含27个潜在的天冬酰胺（N）-连接糖基化位点，并且在第1325 - 1350位有一段高度疏水的残基。
