Duarte M, Laude H
I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, France.
J Gen Virol. 1994 May;75 ( Pt 5):1195-200. doi: 10.1099/0022-1317-75-5-1195.
The complete sequence of the spike (S) gene of the Br1/87 isolate of porcine epidemic diarrhoea virus (PEDV) was determined from cDNA clones. The predicted polypeptide was 1383 amino acids long, contained 29 potential N-linked glycosylation sites and showed structural features similar to those of the coronavirus spike protein. The PEDV S protein, like that of the members of the transmissible gastroenteritis virus (TGEV)-related subset, lacks a proteolytic site to yield cleaved amino and carboxy subunits S1 and S2. Viral polypeptide species of the expected M(r), i.e. 170K/190K, were observed in PEDV-infected cells. Sequence comparison confirmed that, within the subset, PEDV was most closely related to the human respiratory coronavirus HCV 229E. However, PEDV S protein has an additional 250 residue N-terminal domain which is absent from HCV 229E and porcine respiratory coronavirus, the respiratory variant of TGEV. Alignment of the S1 regions revealed a second domain of about 90 residues with increased sequence divergence which might possibly express virus-specific determinants.
从cDNA克隆中确定了猪流行性腹泻病毒（PEDV）Br1/87分离株刺突（S）基因的完整序列。预测的多肽长度为1383个氨基酸，包含29个潜在的N-连接糖基化位点，并且显示出与冠状病毒刺突蛋白相似的结构特征。与传染性胃肠炎病毒（TGEV）相关亚组的成员一样，PEDV S蛋白缺乏产生裂解的氨基和羧基亚基S1和S2的蛋白水解位点。在PEDV感染的细胞中观察到预期相对分子质量（M(r)）的病毒多肽种类，即170K/190K。序列比较证实，在该亚组内，PEDV与人类呼吸道冠状病毒HCV 229E关系最为密切。然而，PEDV S蛋白有一个额外的250个残基的N端结构域，HCV 229E和猪呼吸道冠状病毒（TGEV的呼吸道变体）中不存在该结构域。S1区域的比对揭示了一个约90个残基的第二个结构域，其序列差异增加，可能表达病毒特异性决定簇。
