Tanaka I, Kasper C K, Fulcher C A
Scripps Research Institute, La Jolla, CA.
Thromb Res. 1992 Sep 1;67(5):491-503. doi: 10.1016/0049-3848(92)90011-x.
Sensitive, monoclonal antibody-based, enzyme-linked immunosorbent assays (ELISAs) for plasma factor VIII (FVIII) antigen have been developed. The assays used five monoclonal anti-FVIII antibodies with known epitopes in four different pairwise combinations. The sensitivity of the assays was increased by use of the ELISA amplification method described by Bobrow et. al. (J. Immunol Methods 125:279, 1989). These assays can distinguish between CRM positive, CRM reduced and CRM negative hemophilia A. A total of 94 plasma samples from 78 different patients were screened and generally consistent results were obtained among the different assays. Such assays are potentially useful in the phenotypic and genotypic analysis of hemophilia A.
已经开发出用于血浆因子VIII（FVIII）抗原的基于单克隆抗体的灵敏酶联免疫吸附测定（ELISA）。这些测定使用了四种不同的两两组合中具有已知表位的五种单克隆抗FVIII抗体。通过使用Bobrow等人（《免疫学方法杂志》125:279，1989）描述的ELISA扩增方法提高了测定的灵敏度。这些测定可以区分CRM阳性、CRM降低和CRM阴性的A型血友病。对来自78名不同患者的94份血浆样本进行了筛查，不同测定之间获得了总体一致的结果。此类测定在A型血友病的表型和基因型分析中可能有用。
