Tackaberry E S, Ganz P R, Rock G
J Immunol Methods. 1987 May 4;99(1):59-66. doi: 10.1016/0022-1759(87)90032-9.
Hemophilia A is a congenital bleeding disorder which is characterized by a functional deficiency of the coagulation protein factor VIII. We have developed sensitive enzyme-linked immunosorbent assays (ELISAs) for measuring the antigenic reactivity of factor VIII. The assays utilize dot immunobinding techniques, commercial monoclonal antibodies, and a detection system enhanced by the interaction of avidin and biotin. The dot immunobinding ELISAs were optimized for measuring factor VIII in normal and hemophilic plasma, and in partially and highly purified preparations of factor VIII. Linear standard curves were established for all samples, defining the range for accurate measurement. Factor VIII was detected at concentrations as low as 0.0005 U/ml, which represents 0.1 pg of protein.
甲型血友病是一种先天性出血性疾病，其特征是凝血蛋白因子VIII功能缺陷。我们开发了灵敏的酶联免疫吸附测定法（ELISA）来测量因子VIII的抗原反应性。这些测定法采用斑点免疫结合技术、商用单克隆抗体以及通过抗生物素蛋白和生物素相互作用增强的检测系统。斑点免疫结合ELISA经过优化，可用于测量正常血浆和血友病血浆以及因子VIII部分纯化和高度纯化制剂中的因子VIII。为所有样品建立了线性标准曲线，确定了准确测量的范围。因子VIII的检测浓度低至0.0005 U/ml，相当于0.1 pg蛋白质。
