Pisegna J R, de Weerth A, Huppi K, Wank S A
Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):296-303. doi: 10.1016/0006-291x(92)91557-7.
The receptors for the brain and gastrointestinal peptide, cholecystokinin, can be classified into CCKA and CCKB subtypes. Having recently cloned the rat CCKB receptor, we used it's cDNA to isolate the human CCKB receptor homologue from brain and stomach which encodes a 447 amino acid protein with 90% identity to both rat CCKB and canine gastrin receptors. Northern hybridization identifies transcripts from stomach, pancreas, brain and gallbladder. The CCKB receptor gene maps to chromosome 11. Expression of the receptor cDNA in COS-7 cells was characteristic of a CCKB receptor subtype pharmacology. These data confirm that we have cloned a novel gene for the human brain and stomach CCKB receptor.
大脑和胃肠肽胆囊收缩素的受体可分为CCKA和CCKB亚型。最近我们克隆了大鼠CCKB受体，并用其cDNA从大脑和胃中分离出人类CCKB受体同源物，该同源物编码一种447个氨基酸的蛋白质，与大鼠CCKB受体和犬胃泌素受体均有90%的同源性。Northern杂交鉴定出胃、胰腺、大脑和胆囊中的转录本。CCKB受体基因定位于11号染色体。受体cDNA在COS-7细胞中的表达具有CCKB受体亚型药理学特征。这些数据证实我们克隆了一个新的人类大脑和胃CCKB受体基因。
