丙型肝炎患者中丙型肝炎病毒E2/NS1蛋白抗体的检测

Detection of antibody to hepatitis C E2/NS1 protein in patients with type C hepatitis.

作者信息

Yokosuka O, Ito Y, Imazeki F, Ohto M, Omata M

机构信息

First Department of Medicine, Chiba University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Nov 30;189(1):565-71. doi: 10.1016/0006-291x(92)91595-h.

DOI:10.1016/0006-291x(92)91595-h

PMID:1280430

Abstract

Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectable in none of 7 acute hepatitis, in 2 of 12 chronic persistent hepatitis, in 3 of 25 chronic active hepatitis, and in 2 of 4 cirrhosis. It was detectable in none of 10 normal subjects. In 3 cases who were positive for the antibody before the interferon treatment, it became undetectable after the treatment. Thus, it seems that the antibody is not a neutralizing antibody and is related to active viral replication.

摘要

丙型肝炎病毒假定的E2/NS1序列在大肠杆菌中作为与麦芽糖结合蛋白的融合蛋白表达。获得了约80 kDa的蛋白质，其中包含38 kDa的E2/NS1蛋白。针对该蛋白的抗体在扩增出该序列的同一份血清中可检测到。在7例急性肝炎患者中均未检测到，在12例慢性持续性肝炎患者中的2例、25例慢性活动性肝炎患者中的3例以及4例肝硬化患者中的2例中可检测到。在10名正常受试者中均未检测到。在3例干扰素治疗前抗体呈阳性的患者中，治疗后抗体变得不可检测。因此，似乎该抗体不是中和抗体，且与病毒的活跃复制有关。