Tanaka T, Tsukiyama-Kohara K, Yamaguchi K, Yagi S, Tanaka S, Hasegawa A, Ohta Y, Hattori N, Kohara M
Liver Unit, Tokyo Metropolitan Komagome Hospital, Japan.
Hepatology. 1994 Jun;19(6):1347-53.
Group I and II hepatitis C virus genotypes were determined by a newly developed serological genotyping assay. This assay detected antibodies against group-specific recombinant proteins in the putative NS4 protein region (amino acid no. 1676-1760) by an enzyme-linked immunosorbent assay. This region of the hepatitis C virus peptide has many group-specific amino acids; fewer than 50% of these amino acids are identical between groups I and II. Genotypes determined by the serological genotyping assay were compared with those determined by a method in which the polymerase chain reaction was used in 91 chronic hepatitis patients. The group-specific polymerase chain reaction was performed within the genome region corresponding to the putative NS5 protein, where the group II hepatitis C virus genome is 57 nucleotides longer than that of group I. Among 91 chronic hepatitis C patients who had positive results in the second-generation hepatitis C virus antibody (core and NS3 region) assay, hepatitis C virus RNA was detected in 80 patients by polymerase chain reaction in the 5' untranslated region and in 78 patients by this group-specific polymerase chain reaction. As a result, in 76 of 91 patients (84%) genotypes determined by the serological genotyping assay showed complete agreement with those determined by the group-specific polymerase chain reaction, and none of the patients revealed a group opposite to that of hepatitis C virus genotype. The detection rate of the serological genotyping assay (89 of 91; 98%) was even higher than that of the polymerase chain reaction assay (78 of 91; 86%).(ABSTRACT TRUNCATED AT 250 WORDS)
通过一种新开发的血清学分型检测法来确定丙型肝炎病毒I型和II型。该检测法通过酶联免疫吸附测定法检测针对假定NS4蛋白区域（第1676 - 1760号氨基酸）中组特异性重组蛋白的抗体。丙型肝炎病毒肽的这一区域有许多组特异性氨基酸；I组和II组之间这些氨基酸的相同率不到50%。将血清学分型检测法确定的基因型与在91例慢性肝炎患者中使用聚合酶链反应的方法所确定的基因型进行比较。在与假定NS5蛋白相对应的基因组区域内进行组特异性聚合酶链反应，其中丙型肝炎病毒II型基因组比I型长57个核苷酸。在第二代丙型肝炎病毒抗体（核心和NS3区域）检测呈阳性的91例慢性丙型肝炎患者中，通过5'非翻译区的聚合酶链反应在80例患者中检测到丙型肝炎病毒RNA，通过这种组特异性聚合酶链反应在78例患者中检测到。结果，在91例患者中的76例（84%）中，血清学分型检测法确定的基因型与组特异性聚合酶链反应确定的基因型完全一致，且没有患者显示出与丙型肝炎病毒基因型相反的类型。血清学分型检测法的检出率（91例中的89例；98%）甚至高于聚合酶链反应检测法（91例中的78例；86%）。（摘要截于250字）
