Araújo Eduardo José de Almeida, Sant'Ana Dd Débora de Mello Gonçales, Molinari Sônia Lucy, de Miranda Neto Marcílio Hubner
Universidade Paranaense (UNIPAR), Umuarama, PR, Brasil.
Arq Neuropsiquiatr. 2003 Jun;61(2A):226-33. doi: 10.1590/s0004-282x2003000200012. Epub 2003 Jun 9.
We carried out this work with the purpose of studying the effects of protein and vitamin B deficiency on the morphologic and quantitative aspects of the myenteric plexus of the descending colon of adult Rattus norvegicus. Twenty-eight rats were divided in two groups, one of them receiving chow with 22% protein level (control) and the other fed with chow having 8% protein level without vitamin B supplementation, during 120 days. Whole-mounts of the descending colon were prepared and stained with Giemsa, NADH-diaphorase and NADPH-diaphorase. The undernourished rats had a body weight 11.84% less than the control group. Relative to the controls, the experimental group had a colonic area 48% smaller, 51.9% less Giemsa-stained neurons, 28.3% less NADH-diaphorase positive neurons and 24.2% less NADPH-diaphorase positive neurons.
我们开展这项工作的目的是研究蛋白质和维生素B缺乏对成年挪威大鼠降结肠肌间神经丛形态学和定量方面的影响。28只大鼠被分为两组，其中一组喂食蛋白质水平为22%的食物（对照组），另一组喂食蛋白质水平为8%且未补充维生素B的食物，持续120天。制备降结肠的整装片，并用吉姆萨染色、NADH-黄递酶和NADPH-黄递酶染色。营养不良的大鼠体重比对照组轻11.84%。相对于对照组，实验组的结肠面积小48%，吉姆萨染色的神经元少51.9%，NADH-黄递酶阳性神经元少28.3%，NADPH-黄递酶阳性神经元少24.2%。
