Sant'ana D de M, Miranda Neto M H, de Souza R R, Molinari S L
Departamento de Ciências Morfofisológicas, Universidade Estadual de Maringá, Brasil.
Arq Neuropsiquiatr. 1997 Dec;55(4):687-95. doi: 10.1590/s0004-282x1997000500003.
We carried out this study with the purpose of contributing on the effects of the proteic desnutrition on the morphological aspects and quantitative analysis of the neurons in the myenteric plexus of the ascending colon of adult Rattus norvegicus. Twenty adult rats were divided into two groups: in one of them, we offered a normal ration with proteic level of 22% (control group) and in the other, a ration with a proteic level of 8% (experiment group) during 120 days. We did the whole-mount preparations for the ascending colon and stained them with the Giemsa technique and the histochemical technique of NADH-diaphorase. The rats with proteic desnutrition showed a body weight, on average, to be 35.1% less than those of the control group, and the colon was on average, 26.8% shorter and 6.7% narrower. Thus, it was to be expected that the colon of animals with proteic desnutrition had a neuronal density 31.62% greater than the rats of the control group. Nevertheless, the difference with the Giemsa technique was on average 18.4%, demonstrating a mean neuronal loss of 13.25%.
我们开展这项研究的目的是探讨蛋白质营养不良对成年挪威大鼠升结肠肌间神经丛中神经元形态学及定量分析的影响。将20只成年大鼠分为两组：一组给予蛋白质水平为22%的正常日粮（对照组），另一组给予蛋白质水平为8%的日粮（实验组），持续120天。对升结肠进行整装制片，并用吉姆萨技术和NADH - 黄递酶组织化学技术进行染色。蛋白质营养不良的大鼠体重平均比对照组低35.1%，结肠平均短26.8%，窄6.7%。因此，可以预期蛋白质营养不良动物的结肠神经元密度比对照组大鼠高31.62%。然而，吉姆萨技术检测的差异平均为18.4%，表明平均神经元损失为13.25%。
