Zanin Sônia Trannin de Mello, Molinari Sonia Lucy, Sant'Ana Débora de Mello Gonçales, de Miranda Neto Marcílio Hubner
Departamento de Ciências Morfofisiológicas, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Arq Neuropsiquiatr. 2003 Sep;61(3A):650-3. doi: 10.1590/s0004-282x2003000400023. Epub 2003 Sep 16.
We aim at contributing with information on the quantitative aspects of the NADH-diaphorase positive myenteric neurons of the jejunum of adult rats subjected to protein desnutrition. Ten rats aging 90 days were divided into two groups: control (n=5, 278 g) and disnurtured (n=5, 280 g). In the following 120 days, the rats from the control group had chow with 22% protein level, and those from the disnurtured group, with 8% protein level. After this period, the control rats weighted 394.4g and the disnurtured 273.5g. The jejunum was subjected to the histochemical technique of the NADH-diaphorase to stain nerve cells in whole-mounts. The neurons found in 80 microscopic fields of both groups were counted. In the control 674.6 neurons were observed, and 1326.8 neurons were counted in the disnurtured group. The low-protein diet did not alter the organization of the neurons, but led to a smaller body growth in the disnurtured animals, preventing neuronal dispersal and leading to a greater density per mm .
我们旨在提供有关蛋白质营养不良成年大鼠空肠中烟酰胺腺嘌呤二核苷酸黄递酶（NADH - 黄递酶）阳性肌间神经元定量方面的信息。将10只90日龄大鼠分为两组：对照组（n = 5，体重278克）和营养不良组（n = 5，体重280克）。在接下来的120天里，对照组大鼠食用蛋白质含量为22%的食物，而营养不良组大鼠食用蛋白质含量为8%的食物。此阶段过后，对照组大鼠体重为394.4克，营养不良组大鼠体重为273.5克。对空肠进行NADH - 黄递酶组织化学技术处理，以对整装神经细胞进行染色。对两组80个显微镜视野中发现的神经元进行计数。对照组观察到674.6个神经元，营养不良组计数到1326.8个神经元。低蛋白饮食并未改变神经元的组织结构，但导致营养不良动物身体生长较小，防止了神经元分散，并导致每毫米密度更大。
