Cho Gyeong Jae, Roh Gu Seob, Kim Hyun Joon, Kim Yoon Sook, Cho Soo Hyun, Choi Won Jun, Paik Won Young, Kang Sang Soo, Choi Wan Sung
Department of Anatomy, College of Medicine, Gyeongsang National University, Korea.
J Korean Med Sci. 2003 Jun;18(3):402-8. doi: 10.3346/jkms.2003.18.3.402.
Placental development requires extensive angiogenesis and the invasion of the maternal decidua by the trophoblasts. Adequate and organized interaction of vascular endothelial growth factors (VEGF), placenta growth factors (PlGF), and their receptors are essential for a normal development and function of the placenta. In this study, we evaluated the expressions of PlGFs and their receptors, mRNAs by Northern blotting, in situ hybridization and RT-PCR in the normal and pregnancy-induced hypertensive (PIH) placentas. The expression level of PlGF-2 mRNA was lower in the PIH placentas compared to control as assessed by Northern blotting and in situ hybridization. PlGF mRNA was mainly localized to the vasculosyncytial membrane of placental villi and villous stroma. The expression of PlGF receptor-1 (PlGFR-1) was significantly increased in the PIH placentas compared to the normal ones. These results suggest that the alteration of PlGF-2 and PlGFR-1 mRNA expressions in the placenta are related to the pathogenesis of PIH.
胎盘发育需要广泛的血管生成以及滋养层细胞侵入母体蜕膜。血管内皮生长因子（VEGF）、胎盘生长因子（PlGF）及其受体之间充分且有序的相互作用对于胎盘的正常发育和功能至关重要。在本研究中，我们通过Northern印迹法、原位杂交和逆转录聚合酶链反应（RT-PCR）评估了正常胎盘和妊娠高血压综合征（PIH）胎盘组织中PlGF及其受体mRNA的表达情况。通过Northern印迹法和原位杂交评估发现，与对照组相比，PIH胎盘组织中PlGF-2 mRNA的表达水平较低。PlGF mRNA主要定位于胎盘绒毛的血管合体膜和绒毛基质。与正常胎盘相比，PIH胎盘组织中PlGF受体-1（PlGFR-1）的表达显著增加。这些结果表明，胎盘中PlGF-2和PlGFR-1 mRNA表达的改变与PIH的发病机制有关。
