Shen Hongling, Liu Hongyu, Chen Hanping, Guo Yuzhen, Zhang Ming, Xu Xiaoyan, Xiang Wenpei
Laboratory of Perinatology and Eugenic Prenatal Diagnosis, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(1):116-9. doi: 10.1007/BF02828055.
To investigate the expressions of placental growth factor (PLGF) in placenta with hypertensive disorders of pregnancy (HDP), 45 women with HDP and 20 normally pregnant women were studied. Among 45 women with HDP, there were 23 cases of severe preeclampsia and one case of eclampsia. The location and level of PLGF proteins was determined by immunohistochemistry and Western blot. The expression of PLGF mRNA in placenta was assessed by reverse transcriptional-polymerase chain reaction (RT-PCR). The results showed that: (1) The distribution of PLGF in placenta with HDP was similar to normal one, which was mainly in the cytoplasm of villous syncytiotrophoblast and villous stroma; (2) The expression of PLGF protein was significantly decreased in placentas with mild and severe preeclampsia compared to the normal ones (0.3 +/- 0.4 vs 0.6 +/- 0.4, 0.2 +/- 0.5 vs 0.6 +/- 0.4, P < 0.01). There were no differences between the gestational hypertension placenta and normal one (0.5 +/- 0.6 vs 0.6 +/- 0.4, P > 0.05); (3) The transcription levels of the PLGF mRNA in placentas with preeclampsia were significantly lower than in normal groups (3.33 +/- 0.39 vs 4.87 +/- 0.60, 1.97 +/- 0.29 vs 4.87 +/- 0.60, P < 0.01), and no differences were found between the gestational hypertension placenta and normal groups. These findings suggest that the abnormal expression of PLGF in placentas is related to the pathogenesis of HDP.
为研究胎盘生长因子（PLGF）在妊娠高血压疾病（HDP）患者胎盘中的表达情况，本研究纳入了45例HDP患者及20例正常孕妇。45例HDP患者中，重度子痫前期23例，子痫1例。采用免疫组化和蛋白质印迹法检测PLGF蛋白的定位和水平，通过逆转录聚合酶链反应（RT-PCR）评估胎盘组织中PLGF mRNA的表达。结果显示：（1）HDP患者胎盘组织中PLGF的分布与正常胎盘相似，主要位于绒毛合体滋养层细胞及绒毛间质的细胞质中；（2）轻度和重度子痫前期患者胎盘组织中PLGF蛋白表达较正常孕妇显著降低（0.3±0.4 vs 0.6±0.4，0.2±0.5 vs 0.6±0.4，P<0.01）。妊娠期高血压患者胎盘组织与正常孕妇胎盘组织中PLGF蛋白表达差异无统计学意义（0.5±0.6 vs 0.6±0.4，P>0.05）；（3）子痫前期患者胎盘组织中PLGF mRNA转录水平较正常组显著降低（3.33±0.39 vs 4.87±0.60，1.97±0.29 vs 4.87±0.60，P<0.01），妊娠期高血压患者胎盘组织与正常组差异无统计学意义。研究结果提示，胎盘组织中PLGF表达异常与HDP的发病机制有关。
