Sevaljević L, Poznanović G, Ivanović-Matić S
Institute for Biological Research, Belgrade, Yugoslavia.
Toxicology. 1992 Oct;75(1):1-12. doi: 10.1016/0300-483x(92)90120-4.
The capacity of an organophosphate to elicit the acute phase response (APR) was assessed by studying the effects of acute soman intoxication on two major processes which characterize inflammation, cytokine production in macrophages and the expression of acute phase protein (APP) genes in the liver. It was established that the concentration of lymphostimulatory substances secreted by the macrophages of soman-intoxicated rats was increased to a level characteristic of the primary inflammatory reaction. Macrophage activation was followed by increased transcription rates of APP genes and the corresponding mRNA and protein synthesis in the liver. The pattern of the DNA-protein complexes obtained with nuclear extracts and the cis-element of the rat haptoglobin gene in the gel-retardation assay suggested that the molecular events which underlie the expression of APP genes of intoxicated rats are similar to those that occur during the APR. From these findings we concluded that soman intoxication was a metabolic injury which elicited the typical APR.
通过研究急性梭曼中毒对炎症的两个主要特征过程（巨噬细胞中细胞因子的产生以及肝脏中急性期蛋白（APP）基因的表达）的影响，评估了有机磷酸酯引发急性期反应（APR）的能力。结果表明，梭曼中毒大鼠巨噬细胞分泌的淋巴刺激物质浓度增加到了原发性炎症反应的特征水平。巨噬细胞激活后，肝脏中APP基因的转录速率增加，相应的mRNA和蛋白质合成也增加。凝胶阻滞试验中用核提取物和大鼠触珠蛋白基因的顺式元件获得的DNA-蛋白质复合物模式表明，中毒大鼠APP基因表达的分子事件与APR期间发生的事件相似。从这些发现中我们得出结论，梭曼中毒是一种引发典型APR的代谢性损伤。
