Seeman Nadrian C
Department of Chemistry, New York University, New York 10003, USA.
Biochemistry. 2003 Jun 24;42(24):7259-69. doi: 10.1021/bi030079v.
Structural DNA nanotechnology is derived from naturally occurring structures and phenomena in cellular biochemistry. Motifs based on branched DNA molecules are linked together by sticky ends to produce objects, periodic arrays, and nanomechanical devices. The motifs include Holliday junction analogues, double and triple crossover molecules, knots, and parallelograms. Polyhedral catenanes, such as a cube or a truncated octahedron, have been assembled from branched junctions. Stiff motifs have been used to produce periodic arrays, containing topographic features visible in atomic force microscopy; these include deliberately striped patterns and cavities whose sizes can be tuned by design. Deliberately knotted molecules have been assembled. Aperiodic arrangements of DNA tiles can be used to produce assemblies corresponding to logical computation. Both DNA structural transitions and branch migration have been used as the basis for the operation of DNA nanomechanical devices. Structural DNA nanotechnology has been used in a number of applications in biochemistry. An RNA knot has been used to establish the existence of RNA topoisomerase activity. The sequence dependence of crossover isomerization and branch migration at symmetric sites has been established through the use of symmetric immobile junctions. DNA parallelogram arrays have been used to determine the interhelical angles for a variety of DNA branched junctions. The relationship between biochemistry and structural DNA nanotechnology continues to grow.
结构DNA纳米技术源自细胞生物化学中天然存在的结构和现象。基于分支DNA分子的基序通过粘性末端连接在一起，以产生物体、周期性阵列和纳米机械设备。这些基序包括霍利迪连接类似物、双交叉和三交叉分子、结和平行四边形。多面体连环体，如立方体或截角八面体，已由分支连接组装而成。刚性基序已被用于产生周期性阵列，其中包含原子力显微镜中可见的地形特征；这些特征包括特意设计的条纹图案和尺寸可通过设计进行调整的空洞。特意打结的分子已被组装出来。DNA瓦片的非周期性排列可用于产生与逻辑计算相对应的组件。DNA结构转变和分支迁移都已被用作DNA纳米机械设备运行的基础。结构DNA纳米技术已在生物化学的许多应用中得到使用。一个RNA结已被用于证实RNA拓扑异构酶活性的存在。通过使用对称固定连接，已确定了对称位点处交叉异构化和分支迁移的序列依赖性。DNA平行四边形阵列已被用于确定各种DNA分支连接的螺旋间角度。生物化学与结构DNA纳米技术之间的关系在不断发展。
