Fox K R, Higson S L, Scott J E
Department of Physiology and Pharmacology, University of Southampton, U.K.
Eur J Histochem. 1992;36(3):263-70.
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At low concentrations the ligands showed similar footprinting patterns which centred around AT-rich regions with a mild preference for hompolymeric A and T. At higher concentrations the dyes bound to almost all available DNA sites. Models, with and without intercalation are discussed to account for the specific binding.
甲基绿长期以来一直被用作组织化学中的DNA染色剂。通过DNA酶1和微球菌核酸酶足迹法研究了阳离子三苯甲烷染料甲基绿、结晶紫和孔雀石绿与DNA的序列选择性结合。在低浓度下，配体表现出相似的足迹模式，这些模式集中在富含AT的区域，对同聚A和T有轻微偏好。在较高浓度下，染料几乎与所有可用的DNA位点结合。讨论了有或没有嵌入的模型来解释特异性结合。
