Rawson A J
Rheumatology. 1975;6:190-8.
Peripheral blood lymphocytes from some rheumatoid arthritis (RA) patients show a reduced response to PHA when stimulated directly after isolation from blood. Cells which have been preincubated in medium with normal serum for 22 h before exposure to PHA show a relatively enhanced response, which approximates the normal. These findings could be compatible either with the blocking of surface PHA receptors of circulating RA lymphocytes, or with progressive maturation of these cells in vitro. Preincubation does not enhance the relatively reduced response of RA lymphocytes to allogeneic RA lymphocytes in one-way mixed lymphocyte cultures. An increased reduction in response to PHA following irradiation of RA lymphocytes suggests an increased radiosensitivity of the PHA-responsive subpopulation. This abnormally reduced response was not seen if the cells were preincubated in vitro prior to irradiation.
一些类风湿性关节炎（RA）患者的外周血淋巴细胞在从血液中分离后直接受到刺激时，对PHA的反应降低。在暴露于PHA之前，先在含有正常血清的培养基中预孵育22小时的细胞，其反应相对增强，接近正常水平。这些发现可能与循环中RA淋巴细胞表面PHA受体的阻断有关，也可能与这些细胞在体外的逐渐成熟有关。在单向混合淋巴细胞培养中，预孵育并不能增强RA淋巴细胞对同种异体RA淋巴细胞相对降低的反应。RA淋巴细胞照射后对PHA反应的进一步降低表明PHA反应性亚群的放射敏感性增加。如果细胞在照射前在体外进行预孵育，则不会出现这种异常降低的反应。
