嗜热古菌嗜热栖热甲烷菌中与核糖体RNA操纵子相关的7S RNA基因的体外和体内转录。

Transcription in vitro and in vivo of the 7S RNA gene associated with the ribosomal RNA operon in the hyperthermophilic archaeon Methanothermus fervidus.

作者信息

Koller G, Reeve J N, Frey G, Thomm M

机构信息

Lehrstuhl für Mikrobiologie, Universität Regensburg, FRG.

出版信息

FEMS Microbiol Lett. 1992 Nov 1;77(1-3):95-101. doi: 10.1016/0378-1097(92)90138-e.

DOI:10.1016/0378-1097(92)90138-e

PMID:1281131

Abstract

The gene encoding the archaeal 7S RNA in the hyperthermophile Methanothermus fervidus is linked to a tRNA(Ser) and rRNA operon in the arrangement 5'-7S RNA-14nt-tRNA(Ser)-196nt-16S rRNA and the promoter directing transcription of this 7S RNA gene has now been identified. Initiation of transcription of the 7S RNA gene has been shown to occur both in vivo in M. fervidus and in vitro, using a Methanococcus thermolithotrophicus derived cell-free transcription system, at the first G residue within the initiator sequence ATGG, located 6 bp upstream of the 5' end of the 7S RNA coding region. Cotranscription of the 7S RNA and tRNA(Ser) has been demonstrated in vitro.

摘要

嗜热栖热甲烷菌中编码古菌7S RNA的基因与一个tRNA(Ser)和rRNA操纵子相连，其排列顺序为5'-7S RNA-14nt-tRNA(Ser)-196nt-16S rRNA，并且现已鉴定出指导该7S RNA基因转录的启动子。使用源自嗜热栖热甲烷球菌的无细胞转录系统，已证明7S RNA基因的转录起始在嗜热栖热甲烷菌体内和体外均发生在起始序列ATGG中的第一个G残基处，该残基位于7S RNA编码区5'端上游6 bp处。7S RNA和tRNA(Ser)的共转录已在体外得到证实。