Haas E S, Brown J W, Daniels C J, Reeve J N
Department of Microbiology, Ohio State University, Columbus 43210.
Gene. 1990 May 31;90(1):51-9. doi: 10.1016/0378-1119(90)90438-w.
Analysis of gene structure in the extremely thermophilic archaebacterium, Methanothermus fervidus, has revealed the presence of a cluster of stable RNA-encoding genes arranged 5'-7S RNA-tRNA(Ser)-16S rRNA-tRNA(Ala)-23S rRNA-5S rRNA. The genome of M. fervidus contains two rRNA operons but only one operon has the closely linked 7S RNA-encoding gene. The sequences upstream from the two rRNA operons are identical for 206 bp but diverge at the 3' base of the tRNA(Ser) gene. The secondary structures predicted for the M. fervidus 7S, 16S rRNA, tRNA(Ala) and tRNA(Ser) have been compared with those of functionally homologous molecules from moderately thermophilic and mesophilic archaebacteria. A consensus secondary structure for archaebacterial 7S RNAs has been developed which incorporates bases and structural features also conserved in eukaryotic signal-recognition-particle RNAs and eubacterial 4.5S RNAs.
对嗜热古细菌嗜热栖热菌（Methanothermus fervidus）的基因结构分析表明，存在一组稳定的RNA编码基因簇，其排列顺序为5'-7S RNA- tRNA(Ser)-16S rRNA- tRNA(Ala)-23S rRNA-5S rRNA。嗜热栖热菌的基因组包含两个rRNA操纵子，但只有一个操纵子有紧密相连的7S RNA编码基因。两个rRNA操纵子上游的序列在206 bp内是相同的，但在tRNA(Ser)基因的3'端碱基处发生分歧。已将嗜热栖热菌7S、16S rRNA、tRNA(Ala)和tRNA(Ser)预测的二级结构与中度嗜热和嗜温古细菌功能同源分子的二级结构进行了比较。已开发出古细菌7S RNA的共有二级结构，其中包含在真核信号识别颗粒RNA和真细菌4.5S RNA中也保守的碱基和结构特征。
