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嗜热古菌激烈火球菌的生长速率对核糖体RNA转录的调控

Regulation of ribosomal RNA transcription by growth rate of the hyperthermophilic Archaeon, Pyrococcus furiosus.

作者信息

DiRuggiero J, Achenbach L A, Brown S H, Kelly R M, Robb F T

机构信息

Center of Marine Biotechnology, University of Maryland, Baltimore 21202.

出版信息

FEMS Microbiol Lett. 1993 Aug 1;111(2-3):159-64. doi: 10.1111/j.1574-6968.1993.tb06379.x.

DOI:10.1111/j.1574-6968.1993.tb06379.x
PMID:7691680
Abstract

We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluster encoding the 16S and 23S rRNA genes of P. furiosus was cloned and the 1.9 kb region upstream of the 16S rRNA gene was sequenced.

摘要

我们研究了古菌嗜热栖热菌(Pyrococcus furiosus)的单个rRNA基因簇。该菌株在100摄氏度时生长最佳,因此是嗜热菌。在嗜热栖热菌中,16S rRNA的转录在生长速率增加20倍时会在7.5倍的范围内受到调控。克隆了编码嗜热栖热菌16S和23S rRNA基因的单个基因簇,并对16S rRNA基因上游1.9 kb的区域进行了测序。

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