Chen Cui-Xia, Xing Quan-Hua, Liang Chun-Yang, Yu Yuan-Jie, Liang Feng-Shan, Wang Hong-Gang, Wang Zhen-Lin, Wang Bin
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan Xue Bao. 2003 Apr;30(4):341-4.
Southern corn rust (SCR) is a destructive disease in maize. The inbred line Qi319 is highly resistant to southern corn rust. SSR technique was employed to preliminary mapping of the resistance gene. Bulked segregant analysis revealed that two primers, phi 118 and phi 041, amplified polymorphic bands. SSR analysis on populations indicated the two primers were linked to the rust resistance gene, which was mapped on the short arm of chromosome 10. In addition, comparative analysis of the amplification bands among different populations revealed that the amplification products with the same primer in different populations were dissimilar. This result indicates that the genetic background may affect results of gene mapping and tagging. So, it is important to select suitable population to performing molecular marker analysis and gene mapping.
南方玉米锈病(SCR)是玉米中的一种毁灭性病害。自交系齐319对南方玉米锈病具有高度抗性。采用SSR技术对抗性基因进行初步定位。混合分组分析法表明,两个引物phi 118和phi 041扩增出了多态性条带。对群体进行的SSR分析表明,这两个引物与锈病抗性基因连锁,该基因被定位在第10号染色体的短臂上。此外,不同群体间扩增条带的比较分析表明,同一引物在不同群体中的扩增产物不同。这一结果表明,遗传背景可能会影响基因定位和标记的结果。因此,选择合适的群体进行分子标记分析和基因定位非常重要。