打破遗传密码的简并性。
Breaking the degeneracy of the genetic code.
作者信息
Kwon Inchan, Kirshenbaum Kent, Tirrell David A
机构信息
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
J Am Chem Soc. 2003 Jun 25;125(25):7512-3. doi: 10.1021/ja0350076.
A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.
为了探索打破大肠杆菌遗传密码简并性的可行性，构建了一种含有修饰（AAA）反密码子的突变型酵母苯丙氨酸转运RNA（ytRNAPheAAA）。通过使用与ytRNAPheAAA和突变型酵母苯丙氨酰 - tRNA合成酶共转化的大肠杆菌菌株，我们证明了在UUU密码子处L - 3 -（2 - 萘基）丙氨酸（Nal）能有效取代苯丙氨酸（Phe），但在UUC密码子处则不能。
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