不同牙种植体表面结扎诱导种植体周围炎的微生物学和影像学分析

Microbiologic and radiographic analysis of ligature-induced peri-implantitis with different dental implant surfaces.

作者信息

Shibli Jamil Awad, Martins Marilia Compagnoni, Lotufo Roberto Fraga Moreira, Marcantonio Elcio

机构信息

Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, SP, Brazil.

出版信息

Int J Oral Maxillofac Implants. 2003 May-Jun;18(3):383-90.

PMID:12814313

Abstract

PURPOSE

The goal of this study was to evaluate microbiota and radiographic peri-implant bone loss associated with ligature-induced peri-implantitis.

MATERIALS AND METHODS

Thirty-six dental implants with 4 different surfaces (9 commercially pure titanium, 9 titanium plasma-sprayed, 9 hydroxyapatite, and 9 acid-etched) were placed in the edentulous mandibles of 6 dogs. After 3 months with optimal plaque control, abutment connection was performed. On days 0, 20, 40, and 60 after placement of cotton ligatures, both microbiologic samples and periapical radiographs were obtained. The presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia/nigrescens, Campylobacter spp, Capnocytophaga spp, Fusobacterium spp, beta-hemolytic Streptococcus, and Candida spp were evaluated culturally.

RESULTS

P intermedia/nigrescens was detected in 13.89% of implants at baseline and 100% of implants at other periods. P gingivalis was not detected at baseline, but after 20 and 40 days it was detected in 33.34% of implants and at 60 days it was detected in 29.03% of dental implants. Fusobacterium spp was detected in all periods. Streptococci were detected in 16.67% of implants at baseline and in 83.34%, 72.22%, and 77.42% of implants at 20, 40, and 60 days, respectively. Campylobacter spp and Candida spp were detected in low proportions. The total viable count analysis showed no significant differences among surfaces (P = .831), although a significant difference was observed after ligature placement (P < .0014). However, there was no significant qualitative difference, in spite of the difference among the periods. The peri-implant bone loss was not significantly different between all the dental implant surfaces (P = .908).

DISCUSSION AND CONCLUSIONS

These data suggest that with ligature-induced peri-implantitis, both time and periodontal pathogens affect all surfaces equally after 60 days.

摘要

目的

本研究的目的是评估与结扎诱导的种植体周围炎相关的微生物群和种植体周围骨组织的影像学骨吸收情况。

材料与方法

将36颗具有4种不同表面（9颗商业纯钛、9颗钛等离子喷涂、9颗羟基磷灰石和9颗酸蚀）的牙种植体植入6只犬的无牙下颌骨中。在进行了3个月的最佳菌斑控制后，进行基台连接。在放置棉结扎线后的第0、20、40和60天，采集微生物样本并拍摄根尖片。通过培养评估伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌/变黑普氏菌、弯曲杆菌属、二氧化碳嗜纤维菌属、梭杆菌属、β-溶血性链球菌和念珠菌属的存在情况。

结果

中间普氏菌/变黑普氏菌在基线时在13.89%的种植体中被检测到，在其他时期在100%的种植体中被检测到。牙龈卟啉单胞菌在基线时未被检测到，但在20天和40天后在33.34%的种植体中被检测到，在60天时在29.03%的牙种植体中被检测到。梭杆菌属在所有时期均被检测到。链球菌在基线时在16.67%的种植体中被检测到，在20、40和60天时分别在83.34%、72.22%和77.42%的种植体中被检测到。弯曲杆菌属和念珠菌属的检测比例较低。总活菌计数分析显示不同表面之间无显著差异（P = 0.831），尽管在放置结扎线后观察到显著差异（P < 0.0014）。然而，尽管不同时期存在差异，但在定性方面没有显著差异。所有牙种植体表面的种植体周围骨吸收无显著差异（P = 0.908）。