Eke P I, Braswell L D, Fritz M E
Emory University, Yerkes Regional Primate Research Center, Department of Molecular Medicine, Atlanta, GA 30322, USA.
J Periodontol. 1998 Feb;69(2):190-4. doi: 10.1902/jop.1998.69.2.190.
This study examines the microbiota associated with the progression of experimental peri-implantitis and periodontitis induced concurrently in partially edentulous adult monkeys. Root-form and plate-form implants with fixed prosthesis in place for at least 12 months and their corresponding opposite molar teeth were ligated for 6 months. The microbiota in plaque around these ligated dental implants and molars were studied at 0, 1, 2, 3, and 6 months post-ligation. Plaque samples were analyzed by dark-field microscopy and selective and non-selective culture. Putative periodontal pathogens were detected as a major component of the microbiota cultured from plaque samples obtained from experimental peri-implantitis sites. Overall, the types and relative proportions of putative periodontal pathogens in plaque associated with ligature-induced peri-implantitis and ligature-induced periodontitis were similar. Only levels of anaerobic Actinomyces and spirochetes were significantly different between both sites. Spirochete levels were significantly higher at peri-implantitis sites when compared with levels at periodontitis sites after 6 months, and spirochete levels increased significantly between 0 and 6 months post-ligation at implant sites. Levels of spirochetes correlated significantly with probing depth and bone loss at peri-implantitis sites. Overall, Actinomyces levels were higher at periodontitis sites. Porphyromonas species were not detected continuously as part of the peri-implantitis microbiota. In conclusion, this study finds that the microbiota associated with the progression of experimental peri-implantitis and periodontitis occurring concurrently in partially edentulous mouths are similar.
本研究调查了部分缺牙成年猴子同时发生的实验性种植体周围炎和牙周炎进展过程中相关的微生物群。将已就位固定修复体至少12个月的根形和板形种植体及其相应的对侧磨牙结扎6个月。在结扎后0、1、2、3和6个月研究这些结扎的牙种植体和磨牙周围菌斑中的微生物群。通过暗视野显微镜以及选择性和非选择性培养对菌斑样本进行分析。从实验性种植体周围炎部位获得的菌斑样本培养出的微生物群中，主要成分检测到了假定的牙周病原体。总体而言，与结扎诱导的种植体周围炎和结扎诱导的牙周炎相关的菌斑中，假定的牙周病原体的类型和相对比例相似。仅厌氧放线菌和螺旋体的水平在两个部位之间存在显著差异。6个月后，种植体周围炎部位的螺旋体水平与牙周炎部位相比显著更高，并且在种植体部位结扎后0至6个月期间螺旋体水平显著增加。螺旋体水平与种植体周围炎部位的探诊深度和骨吸收显著相关。总体而言，牙周炎部位的放线菌水平更高。卟啉单胞菌属未作为种植体周围炎微生物群的一部分持续检测到。总之，本研究发现，部分无牙口腔中同时发生的实验性种植体周围炎和牙周炎进展过程中相关的微生物群相似。
