Voss R, Schumann K, Nagl W
Division of Cell Biology, The University, Kaiserslautern, Germany.
Plant Mol Biol. 1992 Dec;20(5):781-90. doi: 10.1007/BF00027149.
The expression of phytohemagglutinin (PHA) genes was studied in various tissues during the development of the seeds of Phaseolus coccineus cv. Hammond's Dwarf Scarlet by means of northern hybridization and reverse transcription polymerase chain reaction (RT-PCR). The expression is highly development-dependent, starting in the late stage (cotyledons begin to fill the endosperm cavity, i.e. 17 to 24 days after pollination), and tissue-specific. The highest levels of PHA-mRNA are found in the cotyledons (at a very late stage), much lower levels in the embryo axis. Very low levels could be detected in earlier stages of the endosperm, the integument, the funiculus, and probably also in the embryo suspensor. Some PHA mRNA was found in the cotyledons of dry seeds, indicating the presence of undegraded transcripts in mature seeds.
采用Northern杂交和逆转录聚合酶链反应（RT-PCR）技术，研究了红花菜豆品种哈蒙德矮生猩红种子发育过程中不同组织中植物血凝素（PHA）基因的表达情况。该表达高度依赖于发育阶段，始于后期（子叶开始填充胚乳腔，即授粉后17至24天），且具有组织特异性。PHA-mRNA的最高水平出现在子叶中（在非常后期），在胚轴中的水平要低得多。在胚乳、珠被、珠柄的早期阶段以及可能在胚柄中可检测到极低水平。在干种子的子叶中发现了一些PHA mRNA，表明成熟种子中存在未降解的转录本。
