Riggs C D, Chrispeels M J
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Plant Mol Biol. 1990 Apr;14(4):629-32. doi: 10.1007/BF00027510.
Using methylation sensitive restriction enzymes and DNA gel blot analyses, we have examined DNA methylation patterns at the chromosomal locus harboring the two phytohemagglutinin genes of Phaseolus vulgaris. In cotyledons, where the genes are highly expressed, several sites have become undermethylated, whereas the methylation status of other sites remains constant, both in cotyledons and in other organs. Taken together with measurements of mRNA levels, these studies provide evidence that organ-specific changes in DNA methylation patterns are associated with the organ-specific expression of nuclear genes in a dicotyledonous plant.
我们使用甲基化敏感限制性内切酶和DNA凝胶印迹分析，检测了菜豆两个植物血凝素基因所在染色体位点的DNA甲基化模式。在基因高度表达的子叶中，几个位点的甲基化程度降低，而其他位点的甲基化状态在子叶和其他器官中均保持不变。结合mRNA水平的测量结果，这些研究提供了证据，表明DNA甲基化模式的器官特异性变化与双子叶植物中核基因的器官特异性表达相关。
