从菜豆非种子组织中检测和表征一种凝集素。

Detection and characterization of a lectin from non-seed tissue ofPhaseolus vulgaris.

机构信息

Department of Pure and Applied Biochemistry, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden.

出版信息

Planta. 1984 May;161(3):223-8. doi: 10.1007/BF00982916.

Abstract

The distribution of lectin in various tissues ofPhaseolus vulgaris L. (cv. red) has been investigated using a sensitive solid-phase enzyme immunoassay. Roots, leaves and stems from 3- to 4-week-old plants were screened for their lectin content; low levels could be detected in all organs, with a relative distribution of 37% in roots, 20% in leaves and 43% in stems. The lectin from stemsleaves and roots was then isolated from 5- to 6-week-old plants using extraction, salt fractionation and affinity chromatography on immobilized porcine thyroglobulin. A comparative study of the seed lectin and the lectin isolated from 5- to 6-week-old plants was made using hemagglutination, inhibition of hemagglutination, immunodiffusion, polyacrylamide and agarose electrophoresis. The results showed that lectin isolated from the different tissues was immunologically identical and exhibited the same subunit structure and similar isolectin composition as the seed lectin.

摘要

使用灵敏的固相酶免疫分析法研究了菜豆（cv. 红）各种组织中凝集素的分布。筛选了 3 至 4 周龄植物的根、叶和茎，以检测其凝集素含量；所有器官中都可以检测到低水平的凝集素，相对分布为根 37%、叶 20%、茎 43%。然后，从 5 至 6 周龄的植物中使用提取、盐分级和固定化猪甲状腺球蛋白上的亲和层析，从茎-叶和根中分离出凝集素。使用血凝、血凝抑制、免疫扩散、聚丙烯酰胺和琼脂糖电泳对种子凝集素和从 5 至 6 周龄植物中分离出的凝集素进行了比较研究。结果表明，从不同组织中分离出的凝集素在免疫学上是相同的，并且具有与种子凝集素相同的亚基结构和相似的同工凝集素组成。