Ritter E J, Scott W J, Wilson J G
Teratology. 1975 Dec;12(3):233-8. doi: 10.1002/tera.1420120304.
Pregnant rats were injected ip with 6 mg/kg 6-aminonicotinamide (6-AN) at day 12 of gestation. Embryos removed between 1 and 48 h later had reduced adenosine triphosphate (ATP) concentrations, of about 50% of control values. All fetuses examined near term were malformed. Nicotinamide (NAM, 100 mg/kg) given ip 1 h after 6-AN afforded protection: malformations occurred in only 15% of the survivors; and there was minimal ATP reduction, 15% below control values. NAM given 2 and 4 h after 6-AN produced intermediate ATP concentrations and malformation frequencies. Thus, there was a relation between the embryotoxic and ATP-depressant actions of 6-AN in day 12 rat embryos.
在妊娠第12天，给怀孕大鼠腹腔注射6毫克/千克的6-氨基烟酰胺（6-AN）。在注射后1至48小时取出的胚胎，其三磷酸腺苷（ATP）浓度降低，约为对照值的50%。所有接近足月检查的胎儿均有畸形。在6-AN注射后1小时腹腔注射烟酰胺（NAM，100毫克/千克）可提供保护：仅15%的存活者出现畸形；ATP的减少也微乎其微，比对照值低15%。在6-AN注射后2小时和4小时给予NAM，产生的ATP浓度和畸形频率处于中间水平。因此，6-AN对妊娠第12天大鼠胚胎的胚胎毒性作用和ATP抑制作用之间存在关联。
