Acién Fernández F G, Hall David O, Cañizares Guerrero E, Krishna Rao K, Molina Grima E
Department of Chemical Engineering, University of Almería, E-04071, Almería, Spain.
J Biotechnol. 2003 Jun 26;103(2):137-52. doi: 10.1016/s0168-1656(03)00101-9.
The production of microalga Phaeodactylum tricornutum in an outdoor helical reactor was analysed. The influence of temperature, solar irradiance and air flow rate on the yield of the culture was evaluated. Biomass productivities up to 1.5 g l(-1) per day and photosynthetic efficiency up to 14% were obtained by maintaining the cultures below 30 degrees C, dissolved oxygen levels less than 400% Sat. (with respect to air saturated culture) and controlling the cell density in order to achieve an average irradiance within the culture below 250 microE m(-2) s(-1). Under these conditions, the fluorescence parameter, Fv/Fm, which reflects the maximal efficiency of PSII photochemistry, remained roughly 0.6-0.7 and growth rates up to 0.050 h(-1) were achieved. The average irradiance and the light/dark cycle frequency, were the variables determining the behaviour of the cultures. A hyperbolic relationship between growth rate and biomass productivity with the average irradiance was observed, whereas both biomass productivity and photosynthetic efficiency linearly increased with the light/dark cycle frequencies. Optimum design and operational conditions which maximise the production of P. tricornutum biomass in outdoor helical reactors were determined.
分析了在室外螺旋式反应器中三角褐指藻的生产情况。评估了温度、太阳辐照度和空气流速对培养物产量的影响。通过将培养温度保持在30℃以下、溶解氧水平低于400%饱和度（相对于空气饱和培养物）并控制细胞密度，以使培养物内的平均辐照度低于250 μE m(-2) s(-1)，获得了高达每天1.5 g l(-1)的生物量生产力和高达14%的光合效率。在这些条件下，反映PSII光化学最大效率的荧光参数Fv/Fm大致保持在0.6 - 0.7，生长速率高达0.050 h(-1)。平均辐照度和光/暗循环频率是决定培养物行为的变量。观察到生长速率与生物量生产力与平均辐照度之间呈双曲线关系，而生物量生产力和光合效率均随光/暗循环频率线性增加。确定了使室外螺旋式反应器中三角褐指藻生物量产量最大化的最佳设计和操作条件。
