Jacobsen Anita, Grahl-Nielsen Otto, Magnesen Thorolf
J Appl Phycol. 2012 Jan;24(1):109-116. doi: 10.1007/s10811-011-9655-6. Epub 2011 Feb 16.
Cell density and fatty acid (FA) content of Pavlova lutheri and Chaetoceros muelleri were analysed in a continuous algal production system (250-L bags) with reduced diameter. The cell density and FA content and composition in the algal production system were determined in replicate bags over a period of 5 weeks. The results showed that the cell density and essential FAs increased during the experiment for both species. After 5 weeks the mean cell numbers had increased to 6.0 ± 0.3 × 10(6) cells mL(-1) in the P. lutheri bags and 6.0 ± 0.4 × 10(6) cells mL(-1) in the C. muelleri bags. The content of total FAs increased significantly (p < 0.05) in all of the bags during the experiment. At the end of the experiment the mean total FA content were 2.7 ± 0.3 pg cell(-1) in the P. lutheri bags and 1.8 ± 0.1 pg cell(-1) in the C. muelleri bags. Maximum total FA content registered was 3.0 pg cell(-1) in one of the P. lutheri bags. The content of the essential FAs (ARA, EPA, DHA) increased over time in both of the species. At the end of the experiment the content of EPA (0.6 ± 0.1 pg cell(-1)) and DHA (0.3 ± 0.0 pg cell(-1)) were highest in the P. lutheri bags, while ARA (0.1 ± 0.0 pg cell(-1)) was highest in C. muelleri. EPA and DHA constituted 22% and 11%, respectively, of total FA content in P. lutheri, while ARA constituted 6% of total FA content in C. muelleri. The results from this experiment indicate that flagellates such as P. lutheri perform better in narrow bags with improved light conditions, while diatoms like C. muelleri perform better in wider bags under light limitation. Implications for bivalve hatcheries are discussed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10811-011-9655-6) contains supplementary material, which is available to authorized users.
在一个直径减小的连续藻类生产系统（250升袋子）中，分析了鲁氏巴夫藻（Pavlova lutheri）和牟氏角毛藻（Chaetoceros muelleri）的细胞密度和脂肪酸（FA）含量。在5周的时间里，对藻类生产系统中重复袋子里的细胞密度、FA含量及组成进行了测定。结果表明，在实验过程中，这两个物种的细胞密度和必需脂肪酸均有所增加。5周后，鲁氏巴夫藻袋子中的平均细胞数增加到6.0±0.3×10⁶个细胞/毫升，牟氏角毛藻袋子中的平均细胞数增加到6.0±0.4×10⁶个细胞/毫升。在实验期间，所有袋子中总脂肪酸的含量均显著增加（p＜0.05）。实验结束时，鲁氏巴夫藻袋子中的平均总脂肪酸含量为2.7±0.3皮克/细胞，牟氏角毛藻袋子中的平均总脂肪酸含量为1.8±0.1皮克/细胞。在一个鲁氏巴夫藻袋子中记录到的最大总脂肪酸含量为3.0皮克/细胞。两个物种中必需脂肪酸（ARA、EPA、DHA）的含量均随时间增加。实验结束时，鲁氏巴夫藻袋子中EPA（0.6±0.1皮克/细胞）和DHA（0.3±0.0皮克/细胞）的含量最高，而牟氏角毛藻中ARA（0.1±0.0皮克/细胞）的含量最高。EPA和DHA分别占鲁氏巴夫藻总脂肪酸含量的22%和11%，而ARA占牟氏角毛藻总脂肪酸含量的6%。该实验结果表明，像鲁氏巴夫藻这样的鞭毛虫在光照条件改善的窄袋子中表现更好，而像牟氏角毛藻这样的硅藻在光照受限的宽袋子中表现更好。讨论了对双壳贝类孵化场的影响。电子补充材料：本文的在线版本（doi:10.1007/s10811-011-9655-6）包含补充材料，授权用户可获取。
