Nishihira Jun, Ishibashi Teruo, Fukushima Tsuyoshi, Sun Bailong, Sato Yuji, Todo Satoru
Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Ann N Y Acad Sci. 2003 May;995:171-82. doi: 10.1111/j.1749-6632.2003.tb03220.x.
Macrophage migration inhibitory factor (MIF) functions as a pluripotent cytokine involved in broad-spectrum pathophysiological events in association with inflammation and immune responses. Several reports, including ours, have suggested that MIF is also involved in tumorigenesis; however, its precise role has not been fully investigated. We examined the effectiveness of anti-MIF antibodies on tumor growth and tumor-associated angiogenesis using murine colon cancer cell line, colon 26. We observed a significant inhibition of growth of tumors embedded on the back of BALB/c mice by treatment with anti-MIF antibodies. Next, we implanted a Millipore chamber filled with colon cancer cells in the subcutaneous fascia of the flanks of mice and then treated them with anti-MIF antibodies. We found that angiogenesis was markedly suppressed within the region of the subcutaneous fascia that was in contact with the chamber. To further assess the role of MIF in tumorigenesis, we established MIF transgenic mice, which demonstrated that tumor growth and the associated angiogenesis were significantly enhanced in comparison with control mice.
巨噬细胞移动抑制因子（MIF）作为一种多能细胞因子，参与与炎症和免疫反应相关的广谱病理生理事件。包括我们的研究在内的一些报告表明，MIF也参与肿瘤发生；然而，其确切作用尚未得到充分研究。我们使用小鼠结肠癌细胞系结肠26，研究了抗MIF抗体对肿瘤生长和肿瘤相关血管生成的有效性。我们观察到，用抗MIF抗体处理可显著抑制植入BALB/c小鼠背部的肿瘤生长。接下来，我们将充满结肠癌细胞的密理博小室植入小鼠侧腹的皮下筋膜中，然后用抗MIF抗体处理它们。我们发现，与小室接触的皮下筋膜区域内的血管生成明显受到抑制。为了进一步评估MIF在肿瘤发生中的作用，我们建立了MIF转基因小鼠，结果表明与对照小鼠相比，肿瘤生长和相关血管生成显著增强。
