Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, South Korea.
Fertil Steril. 2010 Dec;94(7):2736-41. doi: 10.1016/j.fertnstert.2010.02.051. Epub 2010 Apr 8.
To evaluate the effectiveness of liquid nitrogen (LN(2)) vapor (indirect contact method) for the storage of human semen.
Experimental study.
University hospital-based fertility center.
PATIENT(S): We evaluated 150 patients with normal sperm parameters.
INTERVENTION(S): Human semen (N = 120) was mixed with Semen Freezing Medium, divided into two groups, frozen, and stored in LN(2) or LN(2) vapor. Frozen semen from each group (n = 40) was thawed after storage for 1 week, 1 month, or 3 months and then analyzed. In the second experiment, semen (n = 30) was divided into four groups, frozen, and stored in LN(2) or stored 7 cm, 12 cm, or 17 cm above the surface of LN(2) for 1 week.
MAIN OUTCOME MEASURE(S): The motility and viability of sperm were evaluated by basic analysis, the morphology was analyzed with use of staining, the DNA integrity was assessed with use of terminal deoxyuridine triphosphate nick end-labeling assays, and active mitochondria were detected with use of rhodamine 123 staining.
RESULT(S): The LN(2) and LN(2) vapor groups did not differ with regard to sperm motility, viability, morphology, DNA integrity, or active mitochondria when the cryostorage periods were compared. Furthermore, the quality of sperm stored within 17 cm of the surface of LN(2) for 1 week did not change.
CONCLUSION(S): The storage of human semen in LN(2) vapor, without direct contact with LN(2), may represent a useful alternative for the effective storage of human semen.
评估液氮(LN2)蒸气(间接接触法)保存人精液的效果。
实验研究。
以大学医院为基础的生育中心。
我们评估了 150 名具有正常精子参数的患者。
将人精液(N=120)与精液冷冻液混合,分成两组,冷冻后分别储存在 LN2 或 LN2 蒸气中。每组(n=40)的冷冻精液在储存 1 周、1 个月或 3 个月后解冻,然后进行分析。在第二个实验中,将精液(n=30)分成四组,冷冻后分别储存在 LN2 中或距离 LN2 表面 7cm、12cm 或 17cm 处 1 周。
通过基础分析评估精子的活力和活力,用染色法分析形态,用末端脱氧尿苷三磷酸缺口末端标记测定法评估 DNA 完整性,用罗丹明 123 染色检测活性线粒体。
当比较冷冻储存期时,LN2 和 LN2 蒸气组之间在精子活力、活力、形态、DNA 完整性或活性线粒体方面没有差异。此外,在 LN2 表面 17cm 内储存 1 周的精子质量没有变化。
将人精液储存在不与 LN2 直接接触的 LN2 蒸气中,可能是有效储存人精液的一种有用替代方法。