Punyatanasakchai Piyaphan, Sophonsritsuk Areephan, Weerakiet Sawaek, Wansumrit Surapee, Chompurat Deonthip
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodhi Hospital, Mahidol University, Bangkok, Thailand.
Fertil Steril. 2008 Nov;90(5):1978-82. doi: 10.1016/j.fertnstert.2007.09.066. Epub 2008 Jan 28.
To compare the effects of cryopreserved sperm in vapor and liquid phases of liquid nitrogen on sperm motility, morphology, and sperm function.
Experimental study.
Andrology laboratory at Ramathibodi Hospital, Thailand.
PATIENT(S): Thirty-eight semen samples with normal motility and sperm count were collected from 38 men who were either patients of an infertility clinic or had donated sperm for research.
INTERVENTION(S): Each semen sample was divided into two aliquots. Samples were frozen with static-phase vapor cooling. One aliquot was plunged into liquid nitrogen (-196 degrees C), and the other was stored in vapor-phase nitrogen (-179 degrees C) for 3 days. Thawing was performed at room temperature.
MAIN OUTCOME MEASURE(S): Motility was determined by using computer-assisted semen analysis, sperm morphology was determined by using eosin-methylene blue staining, and sperm function was determined by using a hemizona binding test.
RESULT(S): Most of the motility parameters of sperm stored in the vapor phase were not significantly different from those stored in the liquid phase of liquid nitrogen, except in amplitude of lateral head displacement. The percentages of normal sperm morphology in both vapor and liquid phases also were not significantly different. There was no significant difference in the number of bound sperm in hemizona between sperm cryopreserved in both vapor and liquid phases of liquid nitrogen.
CONCLUSION(S): Cryopreservation of human sperm in a vapor phase of liquid nitrogen was comparable to cryopreservation in a liquid phase of liquid nitrogen.
比较液氮气相和液相中冷冻保存的精子对精子活力、形态及精子功能的影响。
实验研究。
泰国拉玛蒂博迪医院男科实验室。
从38名男性中收集了38份精子活力和精子计数正常的精液样本，这些男性要么是不孕不育门诊患者，要么是为研究捐献精子者。
每份精液样本分成两份。样本采用静态气相冷却冷冻。一份直接投入液氮（-196℃），另一份在气相氮（-179℃）中保存3天。在室温下解冻。
采用计算机辅助精液分析测定精子活力，采用伊红-亚甲蓝染色测定精子形态，采用半透明带结合试验测定精子功能。
除头部侧向位移幅度外，气相中保存的精子的大多数活力参数与液氮液相中保存的精子相比无显著差异。气相和液相中正常精子形态的百分比也无显著差异。液氮气相和液相中冷冻保存的精子在半透明带中结合精子的数量无显著差异。
人类精子在液氮气相中的冷冻保存与在液氮液相中的冷冻保存效果相当。
