Nardo Luciano G, Nikas George, Makrigiannakis Antonis, Sinatra Fulvia, Nardo Filadelfo
Department of Obstetrics and Gynecology, Center for Reproductive Medicine and Infertility, Department of Biomedical Science, Center of Molecular Biology and Genetics, University of Catania, Catania, Italy.
J Reprod Med. 2003 May;48(5):355-61.
To use histology, scanning electron microscopy (SEM) and immunohistochemistry to examine the expression of pinopodes, and alpha v beta 3 and alpha 4 beta 1 integrins in the same endometrial biopsies.
A prospective observational study. Fifteen consecutive regularly menstruating women with normal hormone profiles were followed for detection of ovulation and then biopsied twice, on postovulatory days luteinizing hormone (LH) +6 and LH +8. Measurements included abundance and development stage of pinopodes and a semiquantitative assessment of intensity of antibody staining for integrins.
All biopsies observed under light microscopy showed an in-phase endometrium. Pinopodes were found developing mostly on day LH +6 and were fully developed on day LH +8. The expression of alpha v beta 3 dimer increased significantly from day LH +6 to day LH +8, while alpha 4 beta 1 dimer did not show any significant change during the same period. Fully developed pinopodes and strong intensity of alpha v beta 3 integrin were the most common findings.
These results indicate a coordinated, synchronous change in morphology and adhesion molecule expression of endometrial epithelial cells on day LH +8 of a normal menstrual cycle.
运用组织学、扫描电子显微镜（SEM）及免疫组织化学方法，检测同一子宫内膜活检组织中胞饮突、αvβ3及α4β1整合素的表达情况。
一项前瞻性观察性研究。连续纳入15名激素水平正常、月经周期规律的女性，监测其排卵情况，随后在排卵后黄体生成素（LH）+6天和LH +8天进行两次活检。测量指标包括胞饮突的丰度和发育阶段，以及整合素抗体染色强度的半定量评估。
所有经光学显微镜观察的活检组织均显示为分泌期子宫内膜。胞饮突大多在LH +6天开始发育，并于LH +8天完全发育成熟。从LH +6天到LH +8天，αvβ3二聚体的表达显著增加，而α4β1二聚体在同一时期未显示出任何显著变化。胞饮突完全发育成熟且αvβ3整合素表达强度高是最常见的结果。
这些结果表明，在正常月经周期的LH +8天，子宫内膜上皮细胞的形态和黏附分子表达发生了协调、同步的变化。
