Seifert P S, Roth I, Schmiedt W, Oelert H, Okada N, Okada H, Bhakdi S
Institute for Medical Microbiology, Johannes Gutenberg University, Mainz, FRG.
Atherosclerosis. 1992 Oct;96(2-3):135-45. doi: 10.1016/0021-9150(92)90060-t.
Blood cells express a cell membrane protein, termed homologous restriction factor 20 (HRF20) and identical to CD59, that can inhibit complement C5b-9 insertion into their membranes. In this report, we investigated by immunohistochemistry whether CD59 was present on cells in human atherosclerotic lesions since membranous C5b-9(m) has been found in lesions. Using a monoclonal anti-CD59 antibody, a cellular CD59 staining pattern was apparent in nearly all lesion specimens. CD59 stain co-localised with macrophage (CD14), T lymphocyte (CD7), endothelial cell (anti-factor VIII related antigen) and smooth muscle cell cytoskeletal-specific antigens (anti-alpha actin and muscle myosin). Endothelial cells always exhibited a more intense stain than the other cell types. CD59 antigen was not localised to any one area of the lesions. Usually CD59-positive cells occurred in clusters rather than as randomly spaced individual cells. CD59 did not stain all cells of the lesion and in particular did not appear to stain all smooth muscle cells. Areas of CD59-negative cells were sometimes observed to exhibit a cellular C5b-9 staining pattern. C5b-9 deposits were also observed in CD59-positive regions. Normal saphenous vein stained strongly for CD59 at the endothelial lining and weakly in the media. Capillaries in atherosclerotic intima always stained strongly for CD59. We conclude that HRF20 is constitutively expressed on endothelium and is under regulatory control in smooth muscle cells. Cellular C5b-9 attack in atherosclerotic lesions is therefore most likely to occur on smooth muscle cells.
血细胞表达一种细胞膜蛋白，称为同源限制因子20（HRF20），与CD59相同，它能抑制补体C5b - 9插入其细胞膜。在本报告中，我们采用免疫组织化学方法研究了人动脉粥样硬化病变细胞中是否存在CD59，因为在病变中已发现膜性C5b - 9（m）。使用单克隆抗CD59抗体，在几乎所有病变标本中都出现了细胞CD59染色模式。CD59染色与巨噬细胞（CD14）、T淋巴细胞（CD7）、内皮细胞（抗因子VIII相关抗原）和平滑肌细胞细胞骨架特异性抗原（抗α - 肌动蛋白和肌球蛋白）共定位。内皮细胞的染色总是比其他细胞类型更强烈。CD59抗原并不局限于病变的任何一个区域。通常CD59阳性细胞成簇出现，而不是随机分布的单个细胞。CD59并未对病变中的所有细胞染色，尤其似乎并未对所有平滑肌细胞染色。有时观察到CD59阴性细胞区域呈现细胞C5b - 9染色模式。在CD59阳性区域也观察到了C5b - 9沉积物。正常大隐静脉在内皮衬里处CD59染色强烈，在中膜处染色较弱。动脉粥样硬化内膜中的毛细血管CD59染色总是很强。我们得出结论，HRF20在内皮细胞上组成性表达，在平滑肌细胞中受调控。因此，动脉粥样硬化病变中的细胞C5b - 9攻击最有可能发生在平滑肌细胞上。
