Konttinen Y T, Ceponis A, Meri S, Vuorikoski A, Kortekangas P, Sorsa T, Sukura A, Santavirta S
Department of Medicine, Helsinki University of Central Hospital, Finland.
Ann Rheum Dis. 1996 Dec;55(12):888-94. doi: 10.1136/ard.55.12.888.
To investigate the role of complement cascade induced damage and protection against it in acute arthritides compared to rheumatoid arthritis and other chronic joint derangements.
C3c, C9, and protectin (CD59) were examined by avidin-biotin-peroxidase complex staining.
Marked deposits of C3c and C9 were found in synovial vasculature and intercellular matrix of the lining in rheumatoid arthritis and in acute arthritides (including bacterial, reactive, and osteoarthritis flare up). Furthermore, protectin was not visible in synovial lining cells and was relatively weakly expressed in stromal and endothelial cells in rheumatoid arthritis; also in acute arthritides protectin expression was weak. In contrast, C3c and C9 deposits were not found in chronic conditions associated with degenerative diseases (osteoarthritis and osteochondritis dissecans) or mechanical causes (patellar luxation and a ruptured meniscus), in which also the protectin expression was prominent in synovial lining, endothelial and some stromal cells.
Activation of the complement in rheumatoid arthritis and in acute arthritides seems to be associated with a decreased protection of synovial cells against cellular effects and lysis mediated by membrane attack complex.
与类风湿关节炎及其他慢性关节紊乱相比，研究补体级联反应诱导的损伤及其防护在急性关节炎中的作用。
采用抗生物素蛋白-生物素-过氧化物酶复合物染色法检测C3c、C9和保护素（CD59）。
在类风湿关节炎及急性关节炎（包括细菌性、反应性和骨关节炎发作）的滑膜血管和衬里的细胞间基质中发现有明显的C3c和C9沉积。此外，在类风湿关节炎的滑膜衬里细胞中未见保护素，在基质和内皮细胞中表达相对较弱；在急性关节炎中保护素表达也较弱。相比之下，在与退行性疾病（骨关节炎和剥脱性骨软骨炎）或机械原因（髌骨脱位和半月板破裂）相关的慢性疾病中未发现C3c和C9沉积，在这些疾病中，保护素在滑膜衬里、内皮和一些基质细胞中表达也很突出。
类风湿关节炎和急性关节炎中补体的激活似乎与滑膜细胞对膜攻击复合物介导的细胞效应和溶解的保护作用降低有关。
