Harvey A J, Ivarie R
AviGenics, Inc., Georgia BioBusiness Center, 111 Riverbend Road, Athens, Georgia 30605, USA.
Poult Sci. 2003 Jun;82(6):927-30. doi: 10.1093/ps/82.6.927.
Increased demand for the production of human biopharmaceuticals in transgenic organisms has led to an intensive effort to develop the hen as a bioreactor producing exogenous proteins in egg white via transgenesis. To date, however, robust methods for transgenic modification of the avian genome have been lacking. We have used a replication-defective retroviral vector derived from avian leukosis virus (ALV) to generate transgenic chickens expressing bacterial beta-lactamase secreted into serum and egg whites through several generations. Expression was driven by the ubiquitous cytomegalovirus (CMV) promoter. Here we describe results from a transgenic lineage (Harvey et al., 2002a,b) in which (1) the transgene was stably transmitted from a G1 founder male (5657) through several generations without silencing, (2) the protein was biologically active, and (3) the level of expression in egg whites was doubled in a G3 homozygote.
对利用转基因生物生产人类生物制药的需求不断增加，促使人们做出巨大努力，将母鸡开发成一种通过转基因在蛋清中产生外源蛋白的生物反应器。然而，迄今为止，一直缺乏对禽类基因组进行转基因修饰的可靠方法。我们使用了一种源自禽白血病病毒（ALV）的复制缺陷型逆转录病毒载体，来培育出几代都能在血清和蛋清中分泌细菌β-内酰胺酶的转基因鸡。表达由普遍存在的巨细胞病毒（CMV）启动子驱动。在此，我们描述了一个转基因品系（Harvey等人，2002a，b）的结果，其中（1）转基因从G1代奠基雄性（5657）稳定遗传至几代且未发生沉默，（2）该蛋白具有生物活性，以及（3）在G3代纯合子中，蛋清中的表达水平翻倍。
