Rapp Jeffrey C, Harvey Alex J, Speksnijder Gordon L, Hu Wei, Ivarie Robert
AviGenics, Inc., Georgia BioBusiness Center, 111 Riverbend Rd., Athens, GA 30605, USA.
Transgenic Res. 2003 Oct;12(5):569-75. doi: 10.1023/a:1025854217349.
We have previously described the expression of a bacterial protein in the egg white of transgenic chickens using a replication-deficient retroviral vector. Here we report the expression of a glycosylated human protein, interferon alpha-2b (hIFN), in the egg white of transgenic hens. The hIFN secreted into the egg white was biologically active as determined by a viral inhibition assay. Purification and carbohydrate analysis of the hIFN expressed in egg white revealed that two of the six major glycosylated hIFN species match the naturally occurring human hIFN glycovariants. These results support the potential of the hen as a bioreactor for the production of commercially valuable, biologically active, and glycosylated proteins in egg white.
我们之前曾描述过利用复制缺陷型逆转录病毒载体在转基因鸡的蛋清中表达一种细菌蛋白。在此，我们报道了一种糖基化的人类蛋白——干扰素α-2b（hIFN）在转基因母鸡蛋清中的表达。通过病毒抑制试验测定，分泌到蛋清中的hIFN具有生物活性。对蛋清中表达的hIFN进行纯化和碳水化合物分析表明，六种主要糖基化hIFN物种中的两种与天然存在的人类hIFN糖变体相匹配。这些结果支持了母鸡作为生物反应器在蛋清中生产具有商业价值、生物活性和糖基化蛋白的潜力。
