Ovcharenko A V, Goliando P B, Zhirnov O P
Vopr Virusol. 1992 Jul-Aug;37(4):207-11.
Mice infected with influenza A/Aichi/2/68 (H3N2) virus or Sendai/960 paramyxovirus were treated by inhalations of aerosol aprotinin, a broad spectrum inhibitor of proteinases. A course of inhalations of finely dispersed aerosol aprotinin including 4 exposures of 35-40 min each daily for 6 days provided respiratory administration of aprotinin in a dose about 100 kallikrein-inhibiting U/mouse per day. In mice treated by aprotinin inhalations, histological examinations showed decreased pulmonary pathology, and their body weights increased as much as in uninfected animals. In the placebo group, the weight decreased until the death of the animals. The protective effect of aprotinin inhalations was 40-80% with inoculation doses 10-100 MLD50/mouse. The treated animals died 2-4 days later than those in the placebo group. The results indicate the expedience of inhalation therapy with aerosol aprotinin in influenza and paramyxovirus respiratory infections.
感染甲型/爱知/2/68（H3N2）流感病毒或仙台/960副粘病毒的小鼠，通过吸入广谱蛋白酶抑制剂抑肽酶气雾剂进行治疗。一个疗程的吸入细分散抑肽酶气雾剂，包括每天4次，每次35 - 40分钟，共6天，通过呼吸道给予的抑肽酶剂量约为每天100卡托普利抑制单位/小鼠。在接受抑肽酶吸入治疗的小鼠中，组织学检查显示肺部病理状况减轻，且它们的体重增长与未感染动物相当。在安慰剂组中，动物体重下降直至死亡。对于接种剂量为10 - 100半数致死剂量（MLD50）/小鼠的情况，抑肽酶吸入的保护效果为40 - 80%。接受治疗的动物比安慰剂组的动物晚2 - 4天死亡。结果表明，在流感和副粘病毒呼吸道感染中，使用抑肽酶气雾剂进行吸入治疗是可行的。
