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发育中小鼠大脑中β-微管蛋白亚型的免疫鉴别。非III类β-微管蛋白的翻译后修饰。

Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins.

作者信息

Linhartová I, Dráber P, Dráberová E, Viklický V

机构信息

Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.

出版信息

Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):919-24. doi: 10.1042/bj2880919.

DOI:10.1042/bj2880919
PMID:1281983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1131974/
Abstract

Individual beta-tubulin isoforms in developing mouse brain were characterized using immunoblotting, after preceding high-resolution isoelectric focusing, with monoclonal antibodies against different structural regions of beta-tubulin. Some of the antibodies reacted with a limited number of tubulin isoforms in all stages of brain development and in HeLa cells. The epitope for the TU-14 antibody was located in the isotype-defining domain and was present on the beta-tubulin isotypes of classes I, II and IV, but absent on the neuron-specific class-III isotype. The data suggest that non-class-III beta-tubulins in mouse brain are substrates for developmentally regulated post-translational modifications and that beta-tubulins of non-neuronal cells are also post-translationally modified.

摘要

在使用针对β-微管蛋白不同结构区域的单克隆抗体进行高分辨率等电聚焦后，通过免疫印迹法对发育中小鼠大脑中的单个β-微管蛋白亚型进行了表征。一些抗体在大脑发育的所有阶段以及HeLa细胞中与有限数量的微管蛋白亚型发生反应。TU-14抗体的表位位于同种型定义域，存在于I、II和IV类β-微管蛋白同种型上，但在神经元特异性III类同种型上不存在。数据表明，小鼠大脑中的非III类β-微管蛋白是发育调控的翻译后修饰的底物，并且非神经元细胞的β-微管蛋白也进行了翻译后修饰。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/dfca0303d111/biochemj00121-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/a1dca45c7e1c/biochemj00121-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/afa5b6ab720d/biochemj00121-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/1029a75e24ed/biochemj00121-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/210165335c3d/biochemj00121-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/d38a1e0d2000/biochemj00121-0220-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/dfca0303d111/biochemj00121-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/a1dca45c7e1c/biochemj00121-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/afa5b6ab720d/biochemj00121-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/1029a75e24ed/biochemj00121-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/210165335c3d/biochemj00121-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/d38a1e0d2000/biochemj00121-0220-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4273/1131974/dfca0303d111/biochemj00121-0221-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Polymorphism of brain tubulin.脑微管蛋白的多态性
Biochemistry. 1981 Apr 28;20(9):2402-9. doi: 10.1021/bi00512a006.
3
High level of tubulin microheterogeneity in the mouse brain.
Neurosci Lett. 1982 Aug 31;31(3):323-8. doi: 10.1016/0304-3940(82)90041-6.
脂肪来源干细胞在基于 PEG 的 3D 基质中生长时会自发表达神经标记物。
Int J Mol Sci. 2023 Jul 28;24(15):12139. doi: 10.3390/ijms241512139.
4
γ-Tubulin in microtubule nucleation and beyond.γ-微管蛋白在微管成核及其他方面的作用
Front Cell Dev Biol. 2022 Sep 1;10:880761. doi: 10.3389/fcell.2022.880761. eCollection 2022.
5
βIII-Tubulin Gene Regulation in Health and Disease.健康与疾病中的βIII微管蛋白基因调控
Front Cell Dev Biol. 2022 Apr 28;10:851542. doi: 10.3389/fcell.2022.851542. eCollection 2022.
6
Dysregulation of Microtubule Nucleating Proteins in Cancer Cells.癌细胞中微管成核蛋白的失调
Cancers (Basel). 2021 Nov 11;13(22):5638. doi: 10.3390/cancers13225638.
7
Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1.蛋白酪氨酸磷酸酶 SHP-1 对小鼠骨髓来源肥大细胞微管起始的调控作用。
Cells. 2019 Apr 11;8(4):345. doi: 10.3390/cells8040345.
8
Class III β-tubulin in normal and cancer tissues.正常组织和癌组织中的III类β微管蛋白
Gene. 2015 Jun 1;563(2):109-14. doi: 10.1016/j.gene.2015.03.061. Epub 2015 Apr 1.
9
Cytoskeleton in mast cell signaling.细胞骨架在肥大细胞信号转导中的作用。
Front Immunol. 2012 May 25;3:130. doi: 10.3389/fimmu.2012.00130. eCollection 2012.
10
Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells.暴露在拟南芥微管原丝模型和细胞中由抗体定义的β-微管蛋白区域。
BMC Plant Biol. 2010 Feb 18;10:29. doi: 10.1186/1471-2229-10-29.
4
Evolution of tubulin heterogeneity during mouse brain development.
Biochimie. 1982 Mar;64(3):165-72. doi: 10.1016/s0300-9084(82)80466-5.
5
Heterogeneity of vertebrate brain tubulins.脊椎动物脑微管蛋白的异质性。
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6
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7
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J Cell Biol. 1982 Feb;92(2):435-42. doi: 10.1083/jcb.92.2.435.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
Microtubule assembly in the absence of added nucleotides.在未添加核苷酸的情况下微管装配。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8. doi: 10.1073/pnas.70.3.765.
10
Some common properties of the protein that incorporates tyrosine as a single unit and the microtubule proteins.将酪氨酸作为单一单元包含在内的蛋白质与微管蛋白的一些共同特性。
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1384-90. doi: 10.1016/0006-291x(74)90351-9.