Weimer P J, Conner A H, Lorenz L F
United States Dairy Forage Research Center, Agricultural Research Service, United States Department of Agriculture, 1925 Linden Drive West, Madison, WI 53706, USA.
Appl Microbiol Biotechnol. 2003 Nov;63(1):29-34. doi: 10.1007/s00253-003-1334-3. Epub 2003 Jun 18.
Residues from the fermentation of cellulose by the anaerobic bacteria Ruminococcus albus (strain 7) or Ruminococcus flavefaciens (strains FD-1 or B34b) containing residual cellulose, bacterial cells and their associated adhesins, were examined for their ability to serve as components of adhesives for plywood fabrication. The residues contained differing amounts of protein (0.4-4.2% of dry weight), but the ratios of monosaccharides recovered following two-stage treatment of the residue with detergent (pH 7) and TFA were similar for all three strains (0.71 glucose:0.18 xylose:0.08 mannose:0.02 galactose), suggesting similarities in exopolysaccharide composition. Three-ply aspen panels prepared with fermentation residues (FR) displayed better shear strength and wood failure under dry conditions than following a vacuum/pressure/soak/dry treatment, but adhesive properties were inferior to those prepared with conventional phenol-formaldehyde (PF) adhesives. However, panels prepared by incorporating the R. albus 7 FR into PF formulation, at 73% by weight of the total adhesive, exhibited shear strength and wood failure similar to that obtained with PF adhesive alone. Use of residues from fermentations by these bacteria as components of adhesives may add value to biomass fermentations aimed primarily at producing ethanol and other chemical products.
对由厌氧细菌白瘤胃球菌（菌株7）或黄瘤胃球菌（菌株FD - 1或B34b）发酵纤维素产生的残留物进行了研究，这些残留物含有残留纤维素、细菌细胞及其相关黏附素，考察了它们作为胶合板制造用胶粘剂组分的能力。这些残留物含有不同量的蛋白质（占干重的0.4 - 4.2%），但用去污剂（pH 7）和三氟乙酸对残留物进行两阶段处理后回收的单糖比例，对于所有三种菌株而言是相似的（葡萄糖∶木糖∶甘露糖∶半乳糖 = 0.71∶0.18∶0.08∶0.02），这表明胞外多糖组成具有相似性。用发酵残留物（FR）制备的三层白杨木面板在干燥条件下显示出比经过真空/压力/浸泡/干燥处理后更好的抗剪强度和木材破坏率，但胶粘剂性能不如用传统酚醛（PF）胶粘剂制备的面板。然而，通过将白瘤胃球菌7的FR以占胶粘剂总重量73%的比例掺入PF配方中制备的面板，其抗剪强度和木材破坏率与单独使用PF胶粘剂时相似。将这些细菌发酵产生的残留物用作胶粘剂组分，可能会为主要旨在生产乙醇和其他化学产品的生物质发酵增加价值。
