Kus Laura, Borys Ewa, Ping Chu Ya, Ferguson Shawn M, Blakely Randy D, Emborg Marina E, Kordower Jeffrey H, Levey Allan I, Mufson Elliott J
Department of Neurological Sciences, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
J Comp Neurol. 2003 Aug 25;463(3):341-57. doi: 10.1002/cne.10759.
A mouse monoclonal antibody (clone 62-2E8) raised against a human recombinant high-affinity choline transporter (CHT)-glutathione-S-transferase fusion protein was used to determine the distribution of immunoreactive profiles containing this protein in the monkey central nervous system (CNS). Within the monkey telencephalon, CHT-immunoreactive perikarya were found in the striatum, nucleus accumbens, medial septum, vertical and horizontal limb nuclei of the diagonal band, nucleus basalis complex, and the bed nucleus of the stria terminalis. Dense fiber staining was observed within the islands of Calleja, olfactory tubercle, hippocampal complex, amygdala; moderate to light fiber staining was seen in iso- and limbic cortices. CHT-containing fibers were also present in sensory and limbic thalamic nuclei, preoptic and hypothalamic areas, and the floccular lobe of the cerebellum. In the brainstem, CHT-immunoreactive profiles were observed in the pedunculopontine and dorsolateral tegmental nuclei, the Edinger-Westphal, oculomotor, trochlear, trigeminal, abducens, facial, ambiguus, dorsal vagal motor, and hypoglossal nuclei. In the spinal cord, CHT-immunoreactive ventral horn motoneurons were seen in close apposition to intensely immunoreactive C-terminals at the level of the cervical spinal cord. CHT immunostaining revealed a similar distribution of labeled profiles in the aged human brain and spinal cord. Dual fluorescent confocal microscopy revealed that the majority of CHT immunoreactive neurons contained the specific cholinergic marker, choline acetyltransferase, at all levels of the monkey CNS. The present observations indicate that the present CHT antibody labels cholinergic structures within the primate CNS and provides an additional marker for the investigation of cholinergic neuronal function in aging and disease.
一种针对人重组高亲和力胆碱转运体（CHT）-谷胱甘肽-S-转移酶融合蛋白产生的小鼠单克隆抗体（克隆62-2E8），用于确定含该蛋白的免疫反应性轮廓在猴中枢神经系统（CNS）中的分布。在猴端脑内，在纹状体、伏隔核、内侧隔核、斜角带垂直和水平肢核、基底核复合体以及终纹床核中发现了CHT免疫反应性胞体。在Calleja岛、嗅结节、海马复合体、杏仁核内观察到密集的纤维染色；在等皮质和边缘皮质中可见中度至轻度的纤维染色。含CHT的纤维也存在于感觉和边缘丘脑核、视前区和下丘脑区以及小脑绒球叶。在脑干中，在脚桥核和背外侧被盖核、动眼神经副核、动眼神经核、滑车神经核、三叉神经核、展神经核、面神经核、疑核、迷走神经背运动核和舌下神经核中观察到CHT免疫反应性轮廓。在脊髓中，在颈脊髓水平，可见CHT免疫反应性腹角运动神经元与强烈免疫反应性的C末端紧密相邻。CHT免疫染色显示在老年人脑和脊髓中标记轮廓的分布相似。双荧光共聚焦显微镜显示，在猴CNS的所有水平，大多数CHT免疫反应性神经元都含有特异性胆碱能标记物胆碱乙酰转移酶。本观察结果表明，目前的CHT抗体标记了灵长类CNS内的胆碱能结构，并为研究衰老和疾病中的胆碱能神经元功能提供了一个额外的标记物。
