Aller M I, Jones A, Merlo D, Paterlini M, Meyer A H, Amtmann U, Brickley S, Jolin H E, McKenzie A N J, Monyer H, Farrant M, Wisden W
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK.
Genesis. 2003 Jun;36(2):97-103. doi: 10.1002/gene.10204.
The cerebellum maintains balance and orientation, refines motor action, stores motor memories, and contributes to the timing aspects of cognition. We generated two mouse lines for making Cre recombinase-mediated gene disruptions largely confined to adult cerebellar granule cells. For this purpose we chose the GABA(A) receptor alpha6 subunit gene, whose expression marks this cell type. Here we describe mouse lines expressing Cre recombinase generated by 1) Cre knocked into the native alpha6 subunit gene by homologous recombination in embryonic stem cells; and 2) Cre recombined into an alpha6 subunit gene carried on a bacterial artificial chromosome (BAC) genomic clone. The fidelity of Cre expression was tested by crossing the mouse lines with the ROSA26 reporter mice. The particular alpha6BAC clone we identified will be valuable for delivering other gene products to cerebellar granule cells.
小脑维持平衡和定向，优化运动动作，存储运动记忆，并在认知的时间方面发挥作用。我们构建了两个小鼠品系，用于进行主要局限于成年小脑颗粒细胞的Cre重组酶介导的基因破坏。为此，我们选择了GABA(A)受体α6亚基基因，其表达标记了这种细胞类型。在此，我们描述了通过以下方式产生的表达Cre重组酶的小鼠品系：1）通过胚胎干细胞中的同源重组将Cre敲入天然α6亚基基因；2）将Cre重组到细菌人工染色体（BAC）基因组克隆携带的α6亚基基因中。通过将小鼠品系与ROSA26报告基因小鼠杂交来测试Cre表达的保真度。我们鉴定出的特定α6BAC克隆对于将其他基因产物递送至小脑颗粒细胞将具有重要价值。
