Massie Michelle R, Lapoczka Elizabeth M, Boggs Kristy D, Stine Karen E, White Glenn E
Department of Biology/Toxicology, Ashland University, Ashland, OH 44805, USA.
Cell Stress Chaperones. 2003 Spring;8(1):1-7. doi: 10.1379/1466-1268(2003)8<1:ettmis>2.0.co;2.
Historically, sodium azide has been used to anesthetize the nematode Caenorhabditis elegans; however, the mechanism by which it survives this exposure is not understood. In this study, we report that exposure of wild-type C elegans to 10 mM sodium azide for up to 90 minutes confers thermotolerance (defined as significantly increased survival probability [SP] at 37 degrees C) on the animal. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed enhanced Hsp70 expression, whereas Western blot analysis revealed the induction of Hsp16. We also tested the only known C elegans Hsp mutant def-21 (codes for Hsp90), which constitutively enters the stress-resistant state known as the dauer larvae. Daf-21 mutants also acquire sodium azide-induced thermotolerance, whereas 3 non-Hsp, constitutive dauer-forming mutants exhibited a variable response to azide exposure. We conclude that the ability of C elegans to survive exposure to azide is associated with the induction of at least 2 stress proteins.
从历史上看，叠氮化钠一直被用于麻醉线虫秀丽隐杆线虫；然而，其在此暴露下存活的机制尚不清楚。在本研究中，我们报告称，将野生型秀丽隐杆线虫暴露于10 mM叠氮化钠中长达90分钟，可使该动物获得耐热性（定义为在37摄氏度时显著提高的存活概率[SP]）。此外，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示Hsp70表达增强，而蛋白质免疫印迹分析显示Hsp16被诱导。我们还测试了秀丽隐杆线虫中唯一已知的热休克蛋白（Hsp）突变体def-21（编码Hsp90），它会持续进入被称为 dauer 幼虫的抗应激状态。Daf-21突变体也获得了叠氮化钠诱导的耐热性，而3个非Hsp、组成型 dauer 形成突变体对叠氮化物暴露表现出不同的反应。我们得出结论，秀丽隐杆线虫在暴露于叠氮化物后存活的能力与至少2种应激蛋白的诱导有关。
