School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
STAR Protoc. 2021 Feb 4;2(1):100309. doi: 10.1016/j.xpro.2021.100309. eCollection 2021 Mar 19.
The nematode nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A -promoter-driven GFP reporter line [GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using at late L4 larval stage by confocal microscopy. This protocol can also be applied to imaging other cells in . For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).
秀丽隐杆线虫的伤害性 PVD 神经元具有高度有序的树突分支，这使其成为研究树突发育和组织的理想模型。一种由 A -启动子驱动的 GFP 报告基因系 [GFP] 提供了 PVD 解剖结构的全面可视化。在这里，我们通过共聚焦显微镜描述了在晚期 L4 幼虫阶段对 PVD 神经元进行成像的详细过程。该方案也可应用于 中其他细胞的成像。有关此方案的使用和执行的完整详细信息，请参见 Feng 等人。(2020)。
