Espejo Luis S, Freitas Samuel, Hofschneider Vanessa, Chang Leah, Antenor Angelo, Balsa Jonah, Haskins Anne, DeNicola Destiny, Dang Hope, Hamming Sage, Kelser Delaney, Sutphin George L
Molecular & Cellular Biology, University of Arizona, Tucson, AZ, USA, 85721.
bioRxiv. 2024 Aug 13:2023.02.17.529009. doi: 10.1101/2023.02.17.529009.
are an important model system for research on host-microbe interaction. Their rapid life cycle, short lifespan, and transparent body structure allow simple quantification of microbial load and the influence of microbial exposure on host survival. host-microbe interaction studies typically examine group survival and infection severity at fixed timepoints. Here we present an imaging pipeline, Systematic Imaging of Killing Organisms (SICKO), that allows longitudinal characterization of microbes colonizing isolated , enabling dynamic tracking of tissue colonization and host survival in the same animals. Using SICKO, we show that or gut colonization dramatically shortens lifespan and that immunodeficient animals lacking are more susceptible to colonization but display similar colony growth relative to wild type. SICKO opens new avenues for detailed research into bacterial pathogenesis, the benefits of probiotics, and the role of the microbiome in host health.
是宿主-微生物相互作用研究的重要模型系统。它们快速的生命周期、短暂的寿命和透明的身体结构使得对微生物负荷以及微生物暴露对宿主存活的影响进行简单定量成为可能。宿主-微生物相互作用研究通常在固定时间点检查群体存活情况和感染严重程度。在此，我们提出一种成像流程，即杀灭生物的系统成像（SICKO），它能够对定殖在分离体上的微生物进行纵向表征，从而能够在同一动物体内动态追踪组织定殖和宿主存活情况。使用SICKO，我们发现或肠道定殖会显著缩短寿命，并且缺乏的免疫缺陷动物更容易受到定殖影响，但相对于野生型，其菌落生长情况相似。SICKO为深入研究细菌发病机制、益生菌的益处以及微生物群在宿主健康中的作用开辟了新途径。
