Takeda H, Yoshiki A, Nishikawa S, Nishikawa S, Kunisada T, Sakakura T, Amanuma H, Kusakabe M
Laboratory of Gene Technology and Safety, Institute of Physical and Chemical Research, Ibaraki, Japan.
Differentiation. 1992 Oct;51(2):121-7. doi: 10.1111/j.1432-0436.1992.tb00688.x.
The c-kit proto-oncogene encodes a receptor tyrosine kinase and is allelic with the murine white-spoting (W) locus. Although no apparent defects in the brain have been reported in W mutant mice, brain tissue, especially cerebellum, shows a high level of c-kit transcription. In the present study, sites of c-kit expression in the cerebellum were exained by immunohistochemical and in situ hybridization techniques. Immunohistochemistry with a monoclonal antibody against c-Kit protein revealed that the c-Kit protein was localized close to the Purkinje cell soma in the region facing the granular cell layer. Similar distribution of the c-Kit protein was observed in cerebella of mutant mice in which the Purkinje cell (pcd) or the granular cell layer (weaver) is missing. These data suggest that the c-Kit protein is produced not by the Purkinje cell nor by the granular cell but by the cells present in the molecular layer and that the protein is then transported to the region around the Purkinje cell soma. This interpretation was supported by in situ hybridization analysis: cells containing the c-kit transcripts were found only in the molecular layer, while the granular and Purkinje cells were negative.
c-kit原癌基因编码一种受体酪氨酸激酶，与小鼠的白斑（W）位点等位。尽管在W突变小鼠中尚未报道脑有明显缺陷，但脑组织，尤其是小脑，显示出高水平的c-kit转录。在本研究中，通过免疫组织化学和原位杂交技术检查了小脑中c-kit的表达位点。用抗c-Kit蛋白的单克隆抗体进行免疫组织化学分析显示，c-Kit蛋白定位于面向颗粒细胞层区域的浦肯野细胞胞体附近。在浦肯野细胞（pcd）或颗粒细胞层（weaver）缺失的突变小鼠小脑中也观察到了c-Kit蛋白的类似分布。这些数据表明，c-Kit蛋白不是由浦肯野细胞或颗粒细胞产生的，而是由分子层中的细胞产生的，然后该蛋白被转运到浦肯野细胞胞体周围的区域。原位杂交分析支持了这一解释：仅在分子层中发现含有c-kit转录本的细胞，而颗粒细胞和浦肯野细胞为阴性。
